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Application of EMA, Fluorescent Staining and FISH of rDNA in Analysis of Aloe vera (L.) Burm. f. Chromosomes
http://hdl.handle.net/10232/14104
http://hdl.handle.net/10232/14104166c5469-c4dc-4015-8243-befc104f1717
名前 / ファイル | ライセンス | アクション |
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AN00040603_v62_p83-89.pdf (1.3 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2014-12-25 | |||||
タイトル | ||||||
タイトル | Application of EMA, Fluorescent Staining and FISH of rDNA in Analysis of Aloe vera (L.) Burm. f. Chromosomes | |||||
別言語のタイトル | ||||||
その他のタイトル | Aloe vera (L.) Burm. f.の染色体分析におけるEMA法, 蛍光染色及びrDNAのFISHの適用 | |||||
著者 |
JHA, Timir Baran
× JHA, Timir Baran× YAMAMOTO, Masashi |
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著者よみ | ||||||
姓名 | ヤマモト, マサシ | |||||
別言語の著者 | ||||||
姓名 | 山本, 雅 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CMA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DAPI | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | fluorescence in situ hybridization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Indian Aloe | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | karyotype | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 蛍光in situハイブリダイゼーション | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 核型分析 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
要約(Abstract) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Fluorescent banding patterns and the locations of the ribosomal RNA gene (rDNA) of Indian Aloe vera (L.) Burm. f. chromosomes were determined. Root tips were pretreated in 2 mM 8- hydroxyquinoline at 10 to 12℃ for 3h. The chromosome samples were prepared by the enzymatic maceration and air-drying method (EMA). The best preparation, with all chromosomes relatively extended and well spread without cytoplasm, was obtained following enzymatic condition of 0.67% Cellulase Onozuka RS, 0.5% Macerozyme R200 and 0.1% Pectolyase Y-23 for 25 to 35 min at 37°C. No chromomycin A3(CMA) positive or negative bands were detected. Five 4'-6-diamidino-2-phenylindole (DAPI) positive bands, located at centromeric regions in three small and two large chromosomes, were detected with actinomycin D treatment. The 18S-5.8S-25S rDNA sites were detected in telomeric regions of the short arm of one small chromosome and the long arm of two large chromosomes by fluorescence in situ hybridization (FISH). | |||||
要約(Abstract) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Aloe vera (L.) Burm. f. (アロエ) 染色体の蛍光分染及び染色体上でのリボゾームRNA遺伝子(rDNA) の位置の決定を実施した。根端は2mMの8-hydroxyquinolineで10~12℃, 3時間前処理した。染色体標本は酵素解離空気乾燥法(EMA) によって作製した。全染色体が適度に拡がり, 細胞質の無い最も良好な標本は, 以下の条件で得られた;0.67%のCellulase Onozuka RS, 0.5%のMacerozyme R200及び.0.1% Pectolyase Y-23で25~35分, 37℃。Chromomycin A3(CMA) バンドは検出できなかった。Actinomycin D処理を併用すると, 4'-6-diamidino-2-phenylindole (DAPI) +バンドが小染色体の動原部に3か所, 大染色体の端部に2か所の計5か所に出現した。 蛍光in situハイブリダイゼーション(FISH) によって, 18S-5.8S-25S rDNAは1本の小染色体の単腕端部 に1か所, 2本の大染色体の長腕端部に各1か所位置することが明らかとなった。 |
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収録雑誌名 |
鹿兒島大學農學部學術報告=Bulletin of the Faculty of Agriculture, Kagoshima University 巻 62, p. 83-89 |
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作成日 | ||||||
日付 | 2012-03-31 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
ISSN | 04530845 | |||||
NII書誌ID(雑誌) | ||||||
収録物識別子タイプ | NCID | |||||
NC ID | AN00040603 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
NDC | ||||||
主題Scheme | NDC | |||||
主題 | 479 | |||||
公開者・出版者 | ||||||
出版者 | 鹿児島大学 | |||||
公開者よみ | ||||||
公開者よみ | カゴシマ ダイガク | |||||
公開者別名 | ||||||
公開者別名 | Kagoshima University |