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Detection of the ribosomal RNA gene in pear (Pyrus spp.) using fluorescence in situ hybridization
http://hdl.handle.net/10232/18907
http://hdl.handle.net/10232/18907832af265-6adb-42da-87f8-0bd38fbc86dd
名前 / ファイル | ライセンス | アクション |
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YAMAMOTO_J-Japan-Soc-Hort-Sci_v79n4.pdf (206.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-07-29 | |||||
タイトル | ||||||
タイトル言語 | en | |||||
タイトル | Detection of the ribosomal RNA gene in pear (Pyrus spp.) using fluorescence in situ hybridization | |||||
著者 |
山本, 雅史
× 山本, 雅史× 寺上, 伸吾× 山本, 俊哉× 高田, 教臣× 久保, 達也× 冨永, 茂人 |
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言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | chromosome | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | FISH | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | karyotype | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | rDNA | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
要約(Abstract) | ||||||
内容記述タイプ | Other | |||||
内容記述 | The locations of the 18S-5.8S-25S ribosomal RNA gene (rDNA) in the seedlings of Japanese pear [Pyrus pyrifolia (Burm. F.) Nakai] ‘Osa Gold’ and European pear (P. communis L.) ‘La France’ and the 18S ribosomal rDNA in ‘Osa Gold’ were determined using fluorescence in situ hybridization (FISH). 18S-5.8S-25S and 18S rDNA probes were labeled with biotin-16-dUTP. The probes were detected using a fluorescein isothiocyanate (FITC)-avidin conjugate with chromosomes counterstained with propidium iodide (PI). The 18S-5.8S-25S and 18S rDNA sites of ‘Osa Gold’ and the former of ‘La France’ were detected in telomeric positions of six chromosomes. The number and positions of rDNA sites were stable. |
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内容記述言語 | en | |||||
収録雑誌名 |
en : Journal of the Japanese Society for Horticultural Science 巻 79, 号 4, p. 335-339, 発行日 2010 |
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作成日 | ||||||
日付 | 2010-10-01 | |||||
日付タイプ | Issued | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
ISSN | 18823351 | |||||
NII書誌ID(雑誌) | ||||||
収録物識別子タイプ | NCID | |||||
NC ID | AA12177046 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
DOI | https://doi.org/10.2503/jjshs1.79.335 | |||||
権利 | ||||||
権利情報の言語 | ja | |||||
権利情報 | 園芸学会 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
NDC | ||||||
主題Scheme | NDC | |||||
主題 | 625 | |||||
公開者・出版者 | ||||||
出版者言語 | ja | |||||
出版者 | 園芸学会 |