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海洋細菌のタンパク分解酵素に関する研究
http://hdl.handle.net/10232/13705
http://hdl.handle.net/10232/13705421d85ee-49bb-4766-8259-55ce41414ed5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00040498_v24_p149-160.pdf (848.3 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-05-09 | |||||
| タイトル | ||||||
| タイトル | Studies on the proteases of marine bacteria | |||||
| タイトル言語 | en | |||||
| タイトル | ||||||
| タイトル | 海洋細菌のタンパク分解酵素に関する研究 | |||||
| タイトル言語 | ja | |||||
| 著者 |
指原, 信廣
× 指原, 信廣× 坂田, 泰造× 柿本, 大壱 |
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| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Of 34 strains of marine isolates the greater part of them could produce the extracellular protease and half of them had amylase activity. All of them divided into two groups by means of calcium necessity on protease production. The relationship between Ca^{2+} ion and protease activity was studied by using two representative strains I-6 and C-18 for the reason of their high calcium sensitivity among test organisms. The results were as follows: In I-6 strain, the inducible effect on protease activity was observed whenever and immediately after calcium ion supplement. To the contrary, in C-18 strain the effect of Ca^{2+} addition was only observed in logarithmic phase. The protease production of I-6 strain was, if anything, dependent on non-dialysable fractions of polypeptone or yeast extract in medium and that of C-18 strain severely on dialysed fraction of yeast extract. Protease of C-18 strain was heat-labile and had optimal activity at 30 C and at pH 10, while that of I-6 strain was relatively stable below 60 C and having optimal temperature and pH at 50 C and at 10.5, respectively. Metal ions such as Hg^{2+}, KMnO_4 and DFP, specific inhibitor for serine enzymes, strongly inhibited the activities of both enzymes. The metal chelator, EDTA inhibited strongly the enzyme of C-18 strain but partly tha t of I-6 strain. | |||||
| 内容記述言語 | en | |||||
| 収録雑誌名 |
ja : 鹿児島大学水産学部紀要 en : Memoirs of Faculty of Fisheries Kagoshima University 巻 24, p. 149-160, 発行日 1975-11-30 |
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| 作成日 | ||||||
| 日付 | 1975-11-30 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| ISSN | 0453087X | |||||
| NII書誌ID（雑誌） | ||||||
| 収録物識別子タイプ | NCID | |||||
| NC ID | AN00040498 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 660 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 出版者言語 | en | |||||
