{"_buckets": {"deposit": "ec8fb506-dcc8-41d7-b749-95b57863cfea"}, "_deposit": {"created_by": 3, "id": "10843", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "10843"}, "status": "published"}, "_oai": {"id": "oai:ir.kagoshima-u.ac.jp:00010843", "sets": ["40", "5785"]}, "author_link": ["105016", "105014"], "item_7_alternative_title_18": {"attribute_name": "別言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "緑藻Dunaliella tertiolecta Butcherにおけるマンガンの吸収機構"}]}, "item_7_biblio_info_5": {"attribute_name": "収録雑誌名", "attribute_value_mlt": [{"bibliographicIssueNumber": "2", "bibliographicPageEnd": "244", "bibliographicPageStart": "183", "bibliographicVolumeNumber": "34", "bibliographic_titles": [{"bibliographic_title": "鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University"}]}]}, "item_7_date_6": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "1985-12-25"}]}, "item_7_description_4": {"attribute_name": "要約(Abstract)", "attribute_value_mlt": [{"subitem_description": "Although trace metals are present at low concentrations in seawater, algae are capable of\naccumulating such metals in high concentrations against a gradient. In order to clarify the\nuptake mechanism of trace metals, manganese (Mn) uptake from a culture medium by a\neuryhaline alga, Dunaliella tertiolecta BUTCHER was studied.\nD. tertiolecta was affected physiologically by Mn in the medium. Higher growth rates and\nevident elongation in cell length were observed from 0.1 to 1.0 ppm Mn in contrast to other\nconcentrations tested. Crude protein and carbohydrate increased with increasing Mn concentrations with an optimum value at 0.05 to 0.1 ppm Mn. At 1.0 to 10 ppm Mn, protein and\ncarbohydrate synthesis was inhibited. Chlorophyll-a content did not diminish in an Mn\ndeficient medium, but apparently decreased when both Mn and Fe are deficient.\nLive D. tertiolecta cells accumulated Mn from the culture medium subject to environmental\nfactors. Maximum uptake of Mn occured at pH 9.0, 10,000 lux and 20°C temperature. Dead\ncells are unable to take up Mn from the medium and, instead released accumulated Mn to the\ncell exterior. Mn uptake was activated during early exponential growth phase of the batch\nculture. It also synchronized with cell growth, with Mn content reduced at cell division and\nincreased with cell growth. Mn levels inside the cell elevated sharply with increasing levels of\nexogenous Mn from 0.0 to 0.1 ppm. The absorption systems was apparently saturated at 0.1\nppm Mn since further increase up to 1.0 ppm external Mn resulted in virtually no further\nincrease in the Mn content of D. tertiolecta. This saturation kinetics followed Michaelis Menten\nequation. Mn concentration factor in D. tertiolecta was 10-fold at 0.3 to 5.0 ppm and 100-fold at\n0.1 ppm Mn in the medium. Mn uptake was inhibited by DCMU, TPAC, and KCN, and\nstimulated by respiratory substances, glucose. Zn and Cu ions in the medium also inhibited\naccumulation, while Na, K, Mg, Ca, P and Fe accelerated uptake of Mn. In D. tertiolecta,\n72.8% of accumulated Mn was found in the tonoplast, 25.1% in protoplast as free ion or low\nmolecular weight compound, and only 2.1% as firmly bounded to organelles or protoplast.\nThe presence of Mn-stimulated ATPase was confirmed in whole cell homogenates.\nMn-ATPase activity was one-tenth lower than that in Na, K-ATPase, and was strongly\ntemperature dependent with an optimum at 25°C. Optimum pH for the activity was pH 8.0. With increasing Mn concentration, Mn-ATPase activity rose and reached a limiting value at 1.0 mM\nMn (=55 ppm Mn). At higher concentrations, activity slowly diminished and eventually\nvanished at 8.0 mM Mn (=440 ppm Mn). Mn-ATPase was inhibited strongly by oligomycin\nand weakly by ouabine. The activity of Mn-ATPase was detected on cell plasma membranes.\nA highly significant correlation was noted between Mn-ATPase activity and Mn influx at\nconcentrations of 0.0 to 5.0 ppm Mn (P\u003c0.025;r=0.896).\nForm these results, Mn uptake mechanism of D. tertiolecta outlines an active transport\nprocess. Channels or pumps in the cell membrane are activated by energy from Mn-ATPase,\nwhich then takes up Mn from outside into the cell. Accumulated Mn are stored mostly in the\nform of ions or low molecular weight compounds near the cell membrane, while minimum\namounts are transported to organelles i.e., chloroplast. Trace metal accumulation in other\nalgae are likely to follow a similar mechanism found in D. tertiolecta.", "subitem_description_type": "Other"}]}, "item_7_full_name_3": {"attribute_name": "別言語の著者", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "105016", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "野呂, 忠秀"}]}]}, "item_7_publisher_23": {"attribute_name": "公開者・出版者", "attribute_value_mlt": [{"subitem_publisher": "鹿児島大学"}]}, "item_7_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0453087X", "subitem_source_identifier_type": "ISSN"}]}, "item_7_source_id_9": {"attribute_name": "NII書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00040498", "subitem_source_identifier_type": "NCID"}]}, "item_7_subject_15": {"attribute_name": "NDC", "attribute_value_mlt": [{"subitem_subject": "660", "subitem_subject_scheme": "NDC"}]}, "item_7_text_24": {"attribute_name": "公開者よみ", "attribute_value_mlt": [{"subitem_text_value": "カゴシマ ダイガク"}]}, "item_7_text_25": {"attribute_name": "公開者別名", "attribute_value_mlt": [{"subitem_text_value": "Kagoshima University"}]}, "item_7_text_30": {"attribute_name": "NIIタイプ", "attribute_value_mlt": [{"subitem_text_value": "Departmental Bulletin Paper"}]}, "item_7_version_type_14": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "NORO, Tadahide"}], "nameIdentifiers": [{"nameIdentifier": "105014", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-10-31"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AN00040498_v34-2_p183-244.pdf", "filesize": [{"value": "7.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 7700000.0, "url": {"label": "AN00040498_v34-2_p183-244.pdf", "url": "https://ir.kagoshima-u.ac.jp/record/10843/files/AN00040498_v34-2_p183-244.pdf"}, "version_id": "d29e2461-bd1b-41ba-8f96-7eeb6250e7ba"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Mechanism of Manganese Uptake by a Green Alga, Dunaliella tertiolecta Butcher", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Mechanism of Manganese Uptake by a Green Alga, Dunaliella tertiolecta Butcher"}]}, "item_type_id": "7", "owner": "3", "path": ["40", "5785"], "permalink_uri": "http://hdl.handle.net/10232/13331", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-05-07"}, "publish_date": "2012-05-07", "publish_status": "0", "recid": "10843", "relation": {}, "relation_version_is_last": true, "title": ["Mechanism of Manganese Uptake by a Green Alga, Dunaliella tertiolecta Butcher"], "weko_shared_id": 3}