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魚類血液の生化学的研究XV : 血清蛋白質と色素イオンの結合性にみられる種属特異性
http://hdl.handle.net/10232/13884
http://hdl.handle.net/10232/13884bb260c1e-01d8-4f52-bc43-f30079914887
| 名前 / ファイル | ライセンス | アクション |
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AN00040498_v9_p1-15.pdf (7.2 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2015-06-24 | |||||
| タイトル | ||||||
| タイトル | 魚類血液の生化学的研究XV : 血清蛋白質と色素イオンの結合性にみられる種属特異性 | |||||
| タイトル言語 | ja | |||||
| タイトル | ||||||
| タイトル | Biochemical Studies on the Fish Blood XV : On the Species Specificity in the Combinatuib of Serum Protein with Anionic and Cationic Dyes | |||||
| タイトル言語 | en | |||||
| 著者 |
斎藤, 要
× 斎藤, 要 |
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| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Combination of fish serum protein with a cationic dye, methylene blue and with two anionic dyes, methyl orange and bromophenol blue, was studied by equilibrium dialysis, paper electrophoretic analysis and spectrophotometry. The results obtained were summarized as follows. 1) As to the binding ability with methyl orange of various serum proteins, it was ascertained that a considerable amount of methyl orange can be bound with the serum protein of teleosts such as, Scomber tepeinocephalus, Niphon spinosus, Chrysophrys major, Thunnus thynnus, Coryphaena hippurus and Mugil cephalus, and also with the mammalian serum protein (rabbit and bovine), but to the serum protein of elasmobranchs such as Dasyatis akajei, Mustelus griseus, Orectolobus japonicas and Carcharhinus albimarginatus, such binding ability wae barely observed. (Fig. 1). While, the amount of methylene blue bound to serum protein was found to be quite small in any cases. (Fig. 2). In case of teleosts, the binding ability with methyl orange of serum protein varied with kinds of fish and was found in the following order. Bovine (control)＞Scomber tapeinocephalus, Thunnus thynnus＞Chrysophrys major, Mugil cephalus. 2) In the electrophoretic diagrams obtained from the mixture of serum and bromophenol blue, in case of teleosts and control animals there were two peaks of A and B, while in elasmobranchs there was one (A) peak only. (Fig. 5). Peak A (purple) is occasioned by free bromophenol blue and Peak B (blue) is brought forth by the combination of albumin fraction and bromophenol blue. Namely, in case of serum protein of teleosts and control animals there were observed the combination of bromophenol blue with albumin component and a metachromasis according to this combination, but in case of serum protein of elasmobmnchs no such phenomenon could be observed. 3) In the adsorption spectra obtained from the mixture of serum and bromophenol blue, in case of elasmobranchs the adsorption maximum was observed, as in the case of free bromophenol blue (blank), at about 595 mμ, while in teleosts and control animals the maximum was at about 605mμ. (Fig. 6). That is, the serum protein of elasmobranchs does not show metacromasis. In the case in which methylene blue and congo red were used in place of bromophenol blue no such difference in adsorption maximum was found between any samples. (Fig. 7 and 8). From these results it has been ascertained that the serum protein of elasmobranchs, unlike that of teleosts or higher vertebrates, is lacking in albumin component which is rich in cation radicals. | |||||
| 内容記述言語 | en | |||||
| 収録雑誌名 |
ja : 鹿児島大学水産学部紀要 en : Memoirs of Faculty of Fisheries Kagoshima University 巻 9, p. 1-15, 発行日 1960-12-20 |
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| 作成日 | ||||||
| 日付 | 1960-12-20 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| ISSN | 0453087X | |||||
| NII書誌ID（雑誌） | ||||||
| 収録物識別子タイプ | NCID | |||||
| NC ID | AN00040498 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 660 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 出版者言語 | en | |||||
