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植物ホルモン・ブラシノステロイドの内生量調節に関する分子生物学的研究
http://hdl.handle.net/10232/15518
http://hdl.handle.net/10232/155188857e568-f64e-4dde-9110-312bb2eb4f55
| 名前 / ファイル | ライセンス | アクション |
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renken740.pdf (1.2 MB)
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Diss_renken740_Yoshimitsu_20120316.pdf (1.6 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-04-24 | |||||
| タイトル | ||||||
| タイトル | 植物ホルモン・ブラシノステロイドの内生量調節に関する分子生物学的研究 | |||||
| タイトル言語 | ja | |||||
| タイトル | ||||||
| タイトル | Study on the feedback control of brassinosteroid levels in Arabidopsis thaliana | |||||
| タイトル言語 | en | |||||
| タイトル | ||||||
| タイトル | ショクブツ ホルモン ・ ブラシノステロイド ノ ナイセイリョウ チョウセツ ニ カンスル ブンシ セイブツガクテキ ケンキュウ | |||||
| タイトル言語 | ja-Kana | |||||
| 著者 |
吉満, 勇也
× 吉満, 勇也 |
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| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Brassinosteroids (BRs) are a class of plant hormones with a unique polyhydroxy steroid structure, and are essential for growth and development throughout plant life cycle. In addition, BRs ontribute to the enhancement of biotic and abiotic stress tolerance in a variety of plants and promote increased yields in some crop plants, implying that BRs have a potential application in agriculture. To date, many aspects of BR biosynthesis, metabolism and signal transduction pathways have been revealed by both molecular genetic and biochemical research. Through these studies, numerous BR biosynthesis genes have been identified and characterized. Among them, the Arabidopsis DWARF4 (DWF4) that encodes a C-22 hydroxylase has been of interest because it catalyzes multiple key regulatory steps in BR biosynthesis and plays a crucial role in the feedback control of endogenous BR level. However, it still remains to be determined whether DWF4 feedback expression is achieved either by its transcription, post-transcriptional control, or both. Moreover, it is almost unknown whether DWF4 expression is controlled by plant hormones other than BRs. To answer these questions, the intensive analyses using a DWF4::GUS reporter gene were performed to determine the transcriptional behavior of this gene. Several findings regarding the transcriptional control of DWF4 were obtained through these analyses as follows; (1) DWF4 transcription functions as a part of the feedback control machinery that maintains adequate levels of endogenous BRs in response to both their excess and depletion; (2) DWF4 transcription is positively regulated by auxin through the SCFTIR1-mediated auxin signaling; (3) auxin-induced DWF4 transcription is restricted in the elongation zones of both primary and lateral roots as well as the lateral root primordia. Furthermore, through pharmacological analysis, auxin was shown to elongate lateral roots dependently of endogenous BR levels, suggesting that auxin stimulates lateral root growth partly through enhanced BR function, which seems to be caused by auxin-induced DWF4 transcription. Altogether, the results indicate that DWF4 transcription plays dual roles in both the BR homeostasis and the BR-auxin crosstalk that is associated with root growth. In addition to the above findings, I revealed that plasmid DNA is severely degraded and converted into oligonucleotides by tungsten particle that is commonly used as a DNA carrier in biolistic bombardment. Furthermore, I modified the most generally used method of tungsten-DNA adsorption and succeeded to reduce degradation of tungsten bound DNA to the sufficient level for the practical use of this complex on bombardment. | |||||
| 内容記述言語 | en | |||||
| 作成日 | ||||||
| 日付 | 2012-03-16 | |||||
| 日付タイプ | Issued | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 471 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 615 | |||||
| ファイル（説明） | ||||||
| 内容記述 | 博士論文要旨, 博士論文本文 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 出版者言語 | en | |||||
| 学位名 | ||||||
| 学位名の言語 | ja | |||||
| 学位名 | 博士(農学) | |||||
| 学位名 | ||||||
| 学位名の言語 | en | |||||
| 学位名 | Doctor of Philosophy in Agricultural Science | |||||
| 学位授与機関名 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 17701 | |||||
| 学位授与機関名の言語 | ja | |||||
| 学位授与機関名 | 鹿児島大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2012-03-16 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲連研第740号 | |||||
