{"_buckets": {"deposit": "c9f03bd6-db29-4016-85d7-87bcc95097fb"}, "_deposit": {"created_by": 3, "id": "13081", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "13081"}, "status": "published"}, "_oai": {"id": "oai:ir.kagoshima-u.ac.jp:00013081", "sets": ["125"]}, "author_link": ["122012", "122011", "122010"], "item_6_biblio_info_5": {"attribute_name": "収録雑誌名", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "313", "bibliographicPageStart": "305", "bibliographicVolumeNumber": "81", "bibliographic_titles": [{"bibliographic_title": "Journal of the Japanese Society for Horticultural Science"}]}]}, "item_6_date_6": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2012-10-01"}]}, "item_6_description_4": {"attribute_name": "要約(Abstract)", "attribute_value_mlt": [{"subitem_description": "Information on chromosomes is essential for the progress of genetic and biotechnological studies. In this paper, recent progress on studies of chromosome observation in deciduous fruit trees is reviewed. (1) An enzymatic maceration method, preparing good chromosome samples from plants with small chromosomes, was developed for Prunus, Pyrus, Malus, and Diospyros. (2) Some morphologically similar chromosomes could be distinguished by means of the banding technique using fluorochrome staining in Prunus and Pyrus. In addition, the divergence of chromosome configuration seems to be very low or non-existent in view of fluorescent banding patterns in both genera. (3) The number and location of 5S and 18S-5.8S-25S rDNA sites were detected by fluorescent in situ hybridization (FISH) in Prunus, Pyrus, Malus, and Diospyros. The sites of retrotransposons were also visualized by FISH in Pyrus. These results provided fundamental information on the chromosomes. Chromosomal location of the S (self-incompatibility) locus in Malus was also revealed by FISH. In Diospyros, the results of FISH as well as genomic in situ hybridization (GISH) offered new findings on the phylogenetic relationships in this genus and chromosome composition of somatic hybrids.", "subitem_description_type": "Other"}, {"subitem_description": "染色体に関する情報は，育種やバイオテクノロジー研究の進展に不可欠である．その観点から，本論文においては落葉果樹における染色体観察研究の進展についてとりまとめた．概要は以下の通りである．（1）核果類，ナシ，リンゴおよびカキでは，小型染色体における良好な染色体標本作製技術である酵素解離法が確立された．（2）核果類およびナシでは，蛍光色素を用いた分染法によって形態的に似通っている染色体の識別が可能になった．さらに，両種においては，染色体構成（蛍光バンドパターン）の種，品種，個体間差異はほとんど認められなかった．（3）核果類，ナシ，リンゴおよびカキでは，蛍光 in situ ハイブリダイゼーション法（FISH）によって 5S および 18S-5.8S-25S リボゾーム DNA の染色体上での位置が決定された．ナシでは FISH によってレトロトランスポゾン遺伝子の位置も解明された．これらの結果は染色体の基本情報として極めて重要である．さらにリンゴでは，自家不和合性に関する S 遺伝子の染色体上での位置も解明された．カキでは，FISH だけでなくゲノミック in situ ハイブリダイゼーション法（GISH）によっても類縁関係および体細胞雑種の染色体構成に関する新知見が得られている．", "subitem_description_type": "Other"}]}, "item_6_full_name_2": {"attribute_name": "著者よみ", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "122011", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "ヤマモト, マサシ"}]}]}, "item_6_full_name_3": {"attribute_name": "別言語の著者", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "122012", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "山本, 雅史"}]}]}, "item_6_publisher_23": {"attribute_name": "公開者・出版者", "attribute_value_mlt": [{"subitem_publisher": "園芸学会"}]}, "item_6_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.2503/jjshs1.81.305", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "園芸学会"}]}, "item_6_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1882-3351", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_9": {"attribute_name": "NII書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12177046", "subitem_source_identifier_type": "NCID"}]}, "item_6_subject_15": {"attribute_name": "NDC", "attribute_value_mlt": [{"subitem_subject": "625", "subitem_subject_scheme": "NDC"}]}, "item_6_text_30": {"attribute_name": "NIIタイプ", "attribute_value_mlt": [{"subitem_text_value": "Journal Article"}]}, "item_6_version_type_14": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "YAMAMOTO, Masashi"}], "nameIdentifiers": [{"nameIdentifier": "122010", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-10-31"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "YAMAMOTO_J-Japan-Soc-Hort-Sci_v81n4.pdf", "filesize": [{"value": "641.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 641900.0, "url": {"label": "YAMAMOTO_J-Japan-Soc-Hort-Sci_v81n4.pdf", "url": "https://ir.kagoshima-u.ac.jp/record/13081/files/YAMAMOTO_J-Japan-Soc-Hort-Sci_v81n4.pdf"}, "version_id": "a8d1375f-6f45-496f-88f2-59ba1ad7f168"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "CMA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "FISH", "subitem_subject_scheme": "Other"}, {"subitem_subject": "karyotyping", "subitem_subject_scheme": "Other"}, {"subitem_subject": "peach", "subitem_subject_scheme": "Other"}, {"subitem_subject": "pear", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Recent Progress on Studies of Chromosome Observation in Deciduous Fruit Trees", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Recent Progress on Studies of Chromosome Observation in Deciduous Fruit Trees"}]}, "item_type_id": "6", "owner": "3", "path": ["125"], "permalink_uri": "http://hdl.handle.net/10232/18910", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-12-22"}, "publish_date": "2014-12-22", "publish_status": "0", "recid": "13081", "relation": {}, "relation_version_is_last": true, "title": ["Recent Progress on Studies of Chromosome Observation in Deciduous Fruit Trees"], "weko_shared_id": 3}