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Yumoto子宮頸癌細胞の cancer stem-like cells において、S100A16はOct4 と Nanog の発現を制御している
http://hdl.handle.net/10232/00030431
http://hdl.handle.net/10232/00030431a47bcae7-a78b-4662-a79a-fa6532c1d11e
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Diss_冨山_成章_ISK488_2018 (840.3 kB)
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Abstract_冨山_成章_4512810158_2018 (75.0 kB)
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Result_Toyama_Nariaki_iski_488_2018 (764.7 kB)
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Comments_Toyama_Nariaki_isk_488_2018 (377.1 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-01-24 | |||||
| タイトル | ||||||
| タイトル | S100A16 up-regulates Oct4 and Nanog expression in cancer stem-like cells of Yumoto human cervical carcinoma cells | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | Yumoto子宮頸癌細胞の cancer stem-like cells において、S100A16はOct4 と Nanog の発現を制御している | |||||
| 言語 | ja | |||||
| 著者 |
冨山, 成章
× 冨山, 成章 |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | cancer stem‑like cells | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | S100A16 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Oct4 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Nanog | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | sphere formation assay | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約(Abstract) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Cancer stem‑like cells (CSCs), which possess the ability to self‑renewal and are multipotent, are regarded as the cause of tumor formation, recurrence, metastasis and drug resistance. It is necessary to understand the properties of CSCs in order to treat them effectively. It has been previously reported that S100 family proteins, which carry calcium‑binding EF‑hand motifs and are associated with tumorigenic processes, serve crucial roles in maintaining cancer stem‑like properties. S100A16 is upregulated in various types of cancer, including bladder, lung and pancreatic. However, the roles of S100A16 in cancer cells, particularly CSCs, are not clear. The present study investigated the roles of S100A16 in CSCs using the sphere formation assay of Yumoto cells, which are a human cervical carcinoma cell line. The mRNA expression levels were evaluated by reverse transcription‑polymerase chain reaction and the protein expression levels were detected by western blot analysis. Following the sphere formation of Yumoto cells, the mRNA and protein expression level of Oct4, Nanog and S100A16 were increased compared with the control cells. Following transfection with S100A16 small interfering RNA (siRNA), the mRNA and protein expression of Oct4 and Nanog were decreased and the spheroid size was significantly decreased in the sphere formation of Yumoto cells compared with control siRNA treated cells. There was no change in the p53 mRNA expression level, whereas the p53 protein expression level, which was decreased by the sphere formation, was recovered by S100A16 knockdown. In addition, the protein expression levels of Oct4 and Nanog, which were increased in the sphere formation, were decreased by the proteasome inhibitor lactacystin. No differences were observed in the S100A16 protein expression between the presence or absence of lactacystin. These results suggest that S100A16 serves an important role in the CSCs of human cervical carcinoma and is a positive regulator of Oct4 and Nanog. | |||||
| 言語 | en | |||||
| 収録雑誌名 |
en : ONCOLOGY LETTERS 巻 15, p. 9929-9933, 発行日 2018 |
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| 作成日 | ||||||
| 日付 | 2018-12-11 | |||||
| 日付タイプ | Collected | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3892/ol.2018.8568 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | Spandidos Publications | |||||
| 情報源 | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.spandidos-publications.com/ol | |||||
| 関連名称 | ONCOLOGY LETTERS | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 495 | |||||
| ファイル（説明） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨 | |||||
| 言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Spandidos Publications | |||||
| 言語 | en | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | カゴシマ ダイガク | |||||
| 言語 | ja-Kana | |||||
| 備考 | ||||||
| 値 | 【指導教員:武田泰生】 | |||||
| date.appl | ||||||
| 値 | 【学位申請日】2018-07-04 | |||||
| 学位名 | ||||||
| 言語 | ja | |||||
| 学位名 | 博士(医学) | |||||
| 学位名 | ||||||
| 言語 | en | |||||
| 学位名 | Doctor of Philosophy in Medical Science | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 17701 | |||||
| 言語 | ja | |||||
| 学位授与機関名 | 鹿児島大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2018-12-27 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲総研第488号 | |||||
