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遺伝子組み換えトロンボモジュリンの虚血再灌流障害に対する脊髄保護効果
http://hdl.handle.net/10232/00031718
http://hdl.handle.net/10232/00031718c570d389-4743-4925-9ea6-c3e91b0a7fde
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Diss_今釜_逸美_ISK585_2020 (525.5 kB)
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Abstract_今釜_逸美_4505810372_2020 (43.0 kB)
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Result_Imagama_Itsumi_isk_585 (188.2 kB)
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Comments_Imagama_Itsumi_isk_585 (99.0 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-04-14 | |||||
| タイトル | ||||||
| タイトル | Protective effects of recombinant human soluble thrombomodulin on ischemia reperfusion injury of the spinal cord in rabbits | |||||
| タイトル言語 | en | |||||
| タイトル | ||||||
| タイトル | 遺伝子組み換えトロンボモジュリンの虚血再灌流障害に対する脊髄保護効果 | |||||
| タイトル言語 | ja | |||||
| 著者 |
今釜, 逸美
× 今釜, 逸美 |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | descending thoracic and thoracoabdominal aortic aneurysm | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | ischemia reperfusion injury | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | spinal cord | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | recombinant human soluble thrombomodulin | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | OBJECTIVES: Paraplegia is a well-known severe complication of ischemic spinal injury that occurs during surgery for descending thoracic and thoracoabdominal aortic aneurysm. Although several surgical procedures and medications have been used to prevent paraplegia, the strategy for preventing paraplegia has not yet been established. Thrombomodulin (TM), expressed on the plasma membrane of endothelial cells, has been considered to exert cytoprotective effects against ischemia reperfusion injury (IRI). The protective effect of recombinant human soluble (rhs) TM against IRI in the liver and kidneys has been reported; we investigated whether rhsTM can prevent paraplegia in a rabbit ischemic spinal injury model. Moreover, we examined whether rhsTM protects rat pheochromocytoma cell line, PC12, from hypoxia-reoxygenation damage. METHODS: Twenty-two New Zealand white rabbits were intravenously injected with isotonic saline (group C; n = 11) or isotonic saline containing rhsTM (group T; n = 11) before clamping of the aorta just below the branching of the renal artery for 30 minutes. Hind limb motor function was assessed 48 hours after aortic declamping as per the modified Tarlov score. PC12 cells were pretreated without rhsTM (group N) or with 1 μg or 10 μg of rhsTM (group A1 or A2), oxygen and glucose were depleted for 210 minutes, and the cells were incubated for another 24 hours. The cell viability was assessed using the Methyl thiazolyl tetrazolium (MTT) method. RESULTS: Lower limb motor function was significantly better in group T as compared to that in group C in vivo experiment (p < 0.05). The cell viability of the PC12 cells in group A2 was higher than that in group N after the hypoxia-reoxygenation experiment (p < 0.05). CONCLUSIONS: The results suggest that rhsTM may prevent paraplegia due to IRI of the spinal cord during surgical intervention for descending thoracic and thoracoabdominal aortic aneurysm. Itsumi IMAGAMA, Ko-ichi KAWAHARA, Hikari UENO, Ikuro MARUYAMA, Yutaka IMOTO Protective effects of recombinant human soluble thrombomodulin on ischemia reperfusion injury of the spinal cord in rabbits Medical journal of Kagoshima University 73(2021) p.16-22 http://hdl.handle.net/10232/00031676 |
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| 内容記述言語 | en | |||||
| 作成日 | ||||||
| 日付 | 2021-03-10 | |||||
| 日付タイプ | Collected | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 490 | |||||
| ファイル（説明） | ||||||
| 内容記述 | 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 出版者言語 | en | |||||
| 備考 | ||||||
| 備考言語 | ja | |||||
| 備考 | 【指導教員:井本浩】 | |||||
| date.appl | ||||||
| appl | 【学位申請日】2021-02-03 | |||||
| 学位名 | ||||||
| 学位名の言語 | ja | |||||
| 学位名 | 博士(医学) | |||||
| 学位名 | ||||||
| 学位名の言語 | en | |||||
| 学位名 | Doctor of Philosophy in Medical Science | |||||
| 学位授与機関名 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 17701 | |||||
| 学位授与機関名の言語 | ja | |||||
| 学位授与機関名 | 鹿児島大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2021-03-17 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲総研第585号 | |||||
