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Eel Serum Synergistically Enhances Growth of Fish Cell Line GAKS
http://hdl.handle.net/10232/00031871
http://hdl.handle.net/10232/0003187198e9cbcd-969e-48d0-a800-701ab1610766
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00040498_70_p1-8 (1.3 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-04-08 | |||||
| タイトル | ||||||
| タイトル | Eel Serum Synergistically Enhances Growth of Fish Cell Line GAKS | |||||
| 言語 | en | |||||
| 著者 |
TAKUMI, Shota
× TAKUMI, Shota× KOMATSU, Yumiko× DOMEN, Yasuhito× SUMIZAWA, Tomoyuki× SHIOZAKI, Kazuhiro× ISHIKAWA, Manabu× KOMATSU, Masaharu× HAYASHI, Seiichi× YAMADA, Shoji |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | cell culture | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | eel serum | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | fish cell line | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | GAKS | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 要約(Abstract) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Generation of cell lines derived from fish is getting to increase. Generally, mammalian cell line culture needs appropriate media with approximately 5 to 10 % fetal calf serum. However, the optimal culture conditions for fish cell lines have not been established so far. We investigated the effect of exposure to inactivated fish serum on cell culture of goldfish scale cell line GAKS. The 5 % sera from gibel, carp, tilapia, and eel were toxic to GAKS cells. In addition, these fish serum were toxic to not only the GAKS, but also the mammalian cell lines HEK293 and SH-SY5Y. GAKS cells did not proliferate under the culture condition with 5% fish serum. Although it is known that fish serum contains thiaminase, the effect of supplemental addition of vitamin B1 was negligible for the proliferation of GAKS cells. Lower concentrations of fish sera, especially less than 5 % of eel serum stimulated the cell survival of GAKS compared with 5 % of each fish serum. The culture medium containing around 1 % eel serum coupled with 1% FCS was comparable to 5 % FCS conditions for proliferation of GAKS cells. Furthermore, the passage culture was successful under these culture conditions. | |||||
| 言語 | en | |||||
| 収録雑誌名 |
ja : 鹿児島大学水産学部紀要 en : Memoirs of Faculty of Fisheries Kagoshima University 巻 70, p. 1-8, 発行日 2021 |
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| 作成日 | ||||||
| 日付 | 2021 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0453087X | |||||
| NII書誌ID（雑誌） | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00040498 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 660 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 言語 | en | |||||
