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Candida albicansのDMBT1への結合に関与する新規マンノース含有シアロタンパク質アドヘジン
http://hdl.handle.net/10232/00032121
http://hdl.handle.net/10232/00032121d28fd77b-6c24-4d8b-a9cc-c8a41ac4bbd7
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Diss_瀬戸口_大介_ISK671_2022 (17.2 MB)
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Abstract_瀬戸口_大介_4518810208_2022 (75.0 kB)
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Result_SETOGUCHI_Daisuke_isk_671 (106.5 kB)
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Comments_SETOGUCHI_Daisuke_isk_671 (56.7 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-09-20 | |||||
| タイトル | ||||||
| タイトル | A novel mannose-containing sialoprotein adhesin involved in the binding of Candida albicans cells to DMBT1 | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | Candida albicansのDMBT1への結合に関与する新規マンノース含有シアロタンパク質アドヘジン | |||||
| 言語 | ja | |||||
| 著者 |
瀬戸口, 大介
× 瀬戸口, 大介 |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | adhesin | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | binding | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Candida albicans | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | DMBT1 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | sialic acid | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | surface localization | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約(Abstract) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Candida albicans colonizes the oral cavity and causes oral candidiasis and early childhood caries synergistically with cariogenic Streptococcus mutans. Colonization of oral tissues with C. albicans is an essential step in the initiation of these infectious diseases. Deleted in malignant brain tumors 1 (DMBT1), also known as salivary agglutinin or gp-340, belongs to the scavenger receptor cysteine-rich (SRCR) superfamily and has important functions in innate immunity. In the oral cavity, DMBT1 causes microbial adherence to tooth enamel and oral mucosa surfaces, but the adherence of C. albicans to DMBT1 has not been examined. In this study, we investigated the binding of C. albicans to DMBT1 and isolated the fungal components responsible for the binding. Candida albicans specifically bound to DMBT1 and strongly bound to the peptide domain SRCRP2. Binding to SRCRP2 was inhibited by N-acetylneuraminic acid and mannose and by lectins recognizing these sugars. The isolated component had a molecular mass of 25 kDa, contained sialic acid and mannose residues, and inhibited C. albicans binding to SRCRP2. The localization of the 25-kDa protein on the surface of C. albicans cell walls was confirmed by immunostaining and a cell ELISA using an antiserum to the protein, and Western blotting revealed the presence of the 25-kDa protein in the cell wall fraction of C. albicans. These results suggest that the isolated adhesin is localized on the surface of C. albicans cell walls and that sialic acid and mannose residues in the adhesin play a significant role in the binding reaction. This is the peer reviewed version of the following article: Daisuke Setoguchi, Emi Nagata, Takahiko Oho A novel mannose-containing sialoprotein adhesin involved in the binding of Candida albicans cells to DMBT1 Molecular Oral Microbiology 2022;37:154–163, which has been published in final form at [https://doi.org/10.1111/omi.12374]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
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| 言語 | en | |||||
| 作成日 | ||||||
| 日付 | 2022-09-08 | |||||
| 日付タイプ | Collected | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 490 | |||||
| ファイル（説明） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨 | |||||
| 言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | John Wiley & Sons Ltd. | |||||
| 言語 | en | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | カゴシマ ダイガク | |||||
| 言語 | ja-Kana | |||||
| 備考 | ||||||
| 値 | 【指導教員:於保孝彦】 | |||||
| date.appl | ||||||
| 値 | 【学位申請日】2022-07-06 | |||||
| 学位名 | ||||||
| 言語 | ja | |||||
| 学位名 | 博士(歯学) | |||||
| 学位名 | ||||||
| 言語 | en | |||||
| 学位名 | Doctor of Philosophy in Dental Science | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 17701 | |||||
| 言語 | ja | |||||
| 学位授与機関名 | 鹿児島大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2022-09-13 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲総研第671号 | |||||
