WEKO3
アイテム
{"_buckets": {"deposit": "c1f40d40-af61-4f5c-84e8-288591cd22a4"}, "_deposit": {"created_by": 29, "id": "16254", "owners": [29], "pid": {"revision_id": 0, "type": "depid", "value": "16254"}, "status": "published"}, "_oai": {"id": "oai:ir.kagoshima-u.ac.jp:00016254", "sets": ["86"]}, "author_link": ["141338", "141339", "141340"], "item_5_alternative_title_18": {"attribute_name": "別言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "microRNA-99a-5pはSMARCD1を標的としてゲムシタビン抵抗性膀胱癌の細胞老化を誘導する"}]}, "item_5_date_6": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2022-10-07"}]}, "item_5_date_granted_54": {"attribute_name": "学位授与年月日 ", "attribute_value_mlt": [{"subitem_dategranted": "2022-10-18"}]}, "item_5_degree_grantor_53": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "鹿児島大学"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "17701", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_5_degree_name_42": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(医学)Doctor of Philosophy in Medical Science"}]}, "item_5_description_17": {"attribute_name": "ファイル(説明)", "attribute_value_mlt": [{"subitem_description": "博士論文全文", "subitem_description_type": "Other"}, {"subitem_description": "博士論文要旨", "subitem_description_type": "Other"}, {"subitem_description": "最終試験結果の要旨", "subitem_description_type": "Other"}, {"subitem_description": "論文審査の要旨", "subitem_description_type": "Other"}]}, "item_5_description_4": {"attribute_name": "要約(Abstract)", "attribute_value_mlt": [{"subitem_description": "Patients with advanced bladder cancer are generally treated with a combination of chemotherapeutics, including gemcitabine, but the effect is limited due to acquisition of drug resistance. Thus, in this study, we investigated the mechanism of gemcitabine resistance. First, gemcitabine-resistant cells were established and resistance confirmed in vitro and in vivo. Small RNA sequencing analyses were performed to search for miRNAs involved in gemcitabine resistance. miR-99a-5p, selected as a candidate miRNA, was downregulated compared to its parental cells. In gain-of-function studies, miR-99a-5p inhibited cell viabilities and restored sensitivity to gemcitabine. RNA sequencing analysis was performed to find the target gene of miR-99a-5p. SMARCD1 was selected as a candidate gene. Dual-luciferase reporter assays showed that miR-99a-5p directly regulated SMARCD1. Loss-of-function studies conducted with si-RNAs revealed suppression of cell functions and restoration of gemcitabine sensitivity. miR-99a-5p overexpression and SMARCD1 knockdown also suppressed gemcitabine-resistant cells in vivo. Furthermore, β-galactosidase staining showed that miR-99a-5p induction and SMARCD1 suppression contributed to cellular senescence. In summary, tumor-suppressive miR-99a-5p induced cellular senescence in gemcitabine-resistant bladder cancer cells by targeting SMARCD1.\n\nMotoki Tamai, Shuichi Tatarano, Shunsuke Okamura, Wataru Fukumoto, Issei Kawakami, Yoichi Osako, Takashi Sakaguchi, Satoshi Sugita, Masaya Yonemori, Yasutoshi Yamada, Masayuki Nakagawa, Hideki Enokida, Hirofumi Yoshino\nmicroRNA-99a-5p induces cellular senescence in gemcitabine-resistant bladder cancer by targeting SMARCD1\nMolecular Oncology 16(6), 1329-1346 (2022)\nhttps://doi.org/10.1002/1878-0261.13192", "subitem_description_type": "Other"}]}, "item_5_dissertation_number_55": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲総研第673号"}]}, "item_5_full_name_2": {"attribute_name": "著者よみ", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "141339", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "タマイ, モトキ"}]}]}, "item_5_full_name_3": {"attribute_name": "別言語の著者", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "141340", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "TAMAI, Motoki"}]}]}, "item_5_publisher_23": {"attribute_name": "公開者・出版者", "attribute_value_mlt": [{"subitem_publisher": "Federation of European Biochemical Societies / John Wiley \u0026 Sons Ltd"}, {"subitem_publisher": "鹿児島大学"}]}, "item_5_subject_15": {"attribute_name": "NDC", "attribute_value_mlt": [{"subitem_subject": "490", "subitem_subject_scheme": "NDC"}]}, "item_5_text_24": {"attribute_name": "公開者よみ", "attribute_value_mlt": [{"subitem_text_value": "カゴシマ ダイガク"}]}, "item_5_text_25": {"attribute_name": "公開者別名", "attribute_value_mlt": [{"subitem_text_value": "Kagoshima University"}]}, "item_5_text_44": {"attribute_name": "備考", "attribute_value_mlt": [{"subitem_text_value": "【指導教員:榎田英樹】"}]}, "item_5_text_47": {"attribute_name": "date.appl", "attribute_value_mlt": [{"subitem_text_value": "【学位申請日】2022-04-06"}]}, "item_5_version_type_14": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "玉井, 元規"}], "nameIdentifiers": [{"nameIdentifier": "141338", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-11-08"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Diss_玉井_元規_ISK673_2022.pdf", "filesize": [{"value": "2.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2700000.0, "url": {"label": "Diss_玉井_元規_ISK673_2022", "url": "https://ir.kagoshima-u.ac.jp/record/16254/files/Diss_玉井_元規_ISK673_2022.pdf"}, "version_id": "38c78316-efdc-4a44-8610-12e27e660215"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-10-27"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "Abstract_玉井_元規_4518810244_2022.pdf", "filesize": [{"value": "166.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 166600.0, "url": {"label": "Abstract_玉井_元規_4518810244_2022", "url": "https://ir.kagoshima-u.ac.jp/record/16254/files/Abstract_玉井_元規_4518810244_2022.pdf"}, "version_id": "5d0941a0-ce03-44da-ac51-7de728def9ea"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-10-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "Result_TAMAI_Motoki_isk_673.pdf", "filesize": [{"value": "134.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 134800.0, "url": {"label": "Result_TAMAI_Motoki_isk_673", "url": "https://ir.kagoshima-u.ac.jp/record/16254/files/Result_TAMAI_Motoki_isk_673.pdf"}, "version_id": "d9ccd747-f5e7-4f76-80b5-a923eb43c0f0"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-10-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 3, "filename": "Comments_TAMAI_Motoki_isk_673.pdf", "filesize": [{"value": "52.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 52300.0, "url": {"label": "Comments_TAMAI_Motoki_isk_673", "url": "https://ir.kagoshima-u.ac.jp/record/16254/files/Comments_TAMAI_Motoki_isk_673.pdf"}, "version_id": "fd08ccd3-6447-4733-96f2-ce2209e58f1a"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "bladder cancer", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cellular senescence", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gemcitabine resistance", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "miR-99a-5p", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "SMARCD1", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "microRNA-99a-5p induces cellular senescence in gemcitabine-resistant bladder cancer by targeting SMARCD1", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "microRNA-99a-5p induces cellular senescence in gemcitabine-resistant bladder cancer by targeting SMARCD1"}]}, "item_type_id": "5", "owner": "29", "path": ["86"], "permalink_uri": "http://hdl.handle.net/10232/00032139", "pubdate": {"attribute_name": "公開日", "attribute_value": "2022-10-27"}, "publish_date": "2022-10-27", "publish_status": "0", "recid": "16254", "relation": {}, "relation_version_is_last": true, "title": ["microRNA-99a-5p induces cellular senescence in gemcitabine-resistant bladder cancer by targeting SMARCD1"], "weko_shared_id": -1}
microRNA-99a-5p induces cellular senescence in gemcitabine-resistant bladder cancer by targeting SMARCD1
http://hdl.handle.net/10232/00032139
http://hdl.handle.net/10232/00032139ead38c77-0fd5-4f85-a9e7-467b51ed0fb6
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
|
![]() |
|
|
![]() |
|
|
![]() |
|
Item type | 学位論文 / Thesis or Dissertation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-10-27 | |||||
タイトル | ||||||
タイトル | microRNA-99a-5p induces cellular senescence in gemcitabine-resistant bladder cancer by targeting SMARCD1 | |||||
別言語のタイトル | ||||||
その他のタイトル | microRNA-99a-5pはSMARCD1を標的としてゲムシタビン抵抗性膀胱癌の細胞老化を誘導する | |||||
著者 |
玉井, 元規
× 玉井, 元規 |
|||||
著者よみ | ||||||
姓名 | タマイ, モトキ | |||||
別言語の著者 | ||||||
姓名 | TAMAI, Motoki | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | bladder cancer | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | cellular senescence | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | gemcitabine resistance | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | miR-99a-5p | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | SMARCD1 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
要約(Abstract) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Patients with advanced bladder cancer are generally treated with a combination of chemotherapeutics, including gemcitabine, but the effect is limited due to acquisition of drug resistance. Thus, in this study, we investigated the mechanism of gemcitabine resistance. First, gemcitabine-resistant cells were established and resistance confirmed in vitro and in vivo. Small RNA sequencing analyses were performed to search for miRNAs involved in gemcitabine resistance. miR-99a-5p, selected as a candidate miRNA, was downregulated compared to its parental cells. In gain-of-function studies, miR-99a-5p inhibited cell viabilities and restored sensitivity to gemcitabine. RNA sequencing analysis was performed to find the target gene of miR-99a-5p. SMARCD1 was selected as a candidate gene. Dual-luciferase reporter assays showed that miR-99a-5p directly regulated SMARCD1. Loss-of-function studies conducted with si-RNAs revealed suppression of cell functions and restoration of gemcitabine sensitivity. miR-99a-5p overexpression and SMARCD1 knockdown also suppressed gemcitabine-resistant cells in vivo. Furthermore, β-galactosidase staining showed that miR-99a-5p induction and SMARCD1 suppression contributed to cellular senescence. In summary, tumor-suppressive miR-99a-5p induced cellular senescence in gemcitabine-resistant bladder cancer cells by targeting SMARCD1. Motoki Tamai, Shuichi Tatarano, Shunsuke Okamura, Wataru Fukumoto, Issei Kawakami, Yoichi Osako, Takashi Sakaguchi, Satoshi Sugita, Masaya Yonemori, Yasutoshi Yamada, Masayuki Nakagawa, Hideki Enokida, Hirofumi Yoshino microRNA-99a-5p induces cellular senescence in gemcitabine-resistant bladder cancer by targeting SMARCD1 Molecular Oncology 16(6), 1329-1346 (2022) https://doi.org/10.1002/1878-0261.13192 |
|||||
作成日 | ||||||
日付 | 2022-10-07 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
NDC | ||||||
主題Scheme | NDC | |||||
主題 | 490 | |||||
ファイル(説明) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 博士論文全文 | |||||
ファイル(説明) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 博士論文要旨 | |||||
ファイル(説明) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 最終試験結果の要旨 | |||||
ファイル(説明) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 論文審査の要旨 | |||||
公開者・出版者 | ||||||
出版者 | Federation of European Biochemical Societies / John Wiley & Sons Ltd | |||||
公開者・出版者 | ||||||
出版者 | 鹿児島大学 | |||||
公開者よみ | ||||||
公開者よみ | カゴシマ ダイガク | |||||
公開者別名 | ||||||
公開者別名 | Kagoshima University | |||||
備考 | ||||||
備考 | 【指導教員:榎田英樹】 | |||||
date.appl | ||||||
【学位申請日】2022-04-06 | ||||||
学位名 | ||||||
学位名 | 博士(医学)Doctor of Philosophy in Medical Science | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17701 | |||||
学位授与機関名 | 鹿児島大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2022-10-18 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲総研第673号 |