{"_buckets": {"deposit": "c1e9229a-28c3-42e2-a477-123bdcb7c0d1"}, "_deposit": {"created_by": 29, "id": "16540", "owners": [29], "pid": {"revision_id": 0, "type": "depid", "value": "16540"}, "status": "published"}, "_oai": {"id": "oai:ir.kagoshima-u.ac.jp:00016540", "sets": ["86"]}, "author_link": ["142186", "142185", "142187"], "item_5_alternative_title_18": {"attribute_name": "別言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "新興下痢症病原体Escherichia albertiiにおける鞭毛H抗原の多様性と遺伝子型別法の開発"}]}, "item_5_date_6": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2023-03-03"}]}, "item_5_date_granted_54": {"attribute_name": "学位授与年月日 ", "attribute_value_mlt": [{"subitem_dategranted": "2023-03-10"}]}, "item_5_degree_grantor_53": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "鹿児島大学"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "17701", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_5_degree_name_42": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（医学）Doctor of Philosophy in Medical Science"}]}, "item_5_description_17": {"attribute_name": "ファイル（説明）", "attribute_value_mlt": [{"subitem_description": "博士論文全文", "subitem_description_type": "Other"}, {"subitem_description": "博士論文要旨", "subitem_description_type": "Other"}, {"subitem_description": "最終試験結果の要旨", "subitem_description_type": "Other"}, {"subitem_description": "論文審査の要旨", "subitem_description_type": "Other"}]}, "item_5_description_4": {"attribute_name": "要約(Abstract)", "attribute_value_mlt": [{"subitem_description": "Escherichia albertii is a recently recognized human enteropathogen that is closely related to Escherichia coli. As E. albertii sometimes causes outbreaks of gastroenteritis, rapid strain typing systems, such as the O- and H-serotyping systems widely used for E. coli, will be useful for outbreak investigation and surveillance. Although an O-genotyping system has recently been developed, the diversity of E. albertii H-antigens (flagellins) encoded by fliC genes remains to be systematically investigated, and no H-serotyping or genotyping system is currently available. Here, we analyzed the fliC genes of 243 genome-sequenced E. albertii strains and identified 73 sequence types, which were grouped into four clearly distinguishable types designated E. albertii H-genotypes 1–4 (EAHg1–EAHg4). Although there was a clear sign of intraspecies transfer of fliC genes in E. albertii, none of the four E. albertii H-genotypes (EAHgs) were closely related to any of the 53 known E. coli H-antigens, indicating the absence or rare occurrence of interspecies transfer of fliC genes between the two species. Although the analysis of more E. albertii strains will be required to confirm the low level of variation in their fliC genes, this finding suggests that E. albertii may exist in limited natural hosts or environments and/or that the flagella of E. albertii may function in a limited stage(s) in their life cycle. Based on the fliC sequences of the four EAHgs, we developed a multiplex PCR-based H-genotyping system for E. albertii (EAH-genotyping PCR), which will be useful for epidemiological studies of E. albertii infections.\n\nKoji Nakae, Tadasuke Ooka, Koichi Murakami, Yukiko Hara-Kudo, Naoko Imuta, Yasuhiro Gotoh, Yoshitoshi Ogura, Tetsuya Hayashi, Yasuhiro Okamoto and Junichiro Nishi\nDiversification of Escherichia albertii H-Antigens and Development of H-Genotyping PCR\nFrontiers in Microbiology. 12:737979  (2021)\nhttps://doi.org/10.3389/fmicb.2021.737979", "subitem_description_type": "Other"}]}, "item_5_dissertation_number_55": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲総研第696号"}]}, "item_5_full_name_2": {"attribute_name": "著者よみ", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "142186", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "ナカエ, コウジ"}]}]}, "item_5_full_name_3": {"attribute_name": "別言語の著者", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "142187", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "NAKAE, Koji"}]}]}, "item_5_publisher_23": {"attribute_name": "公開者・出版者", "attribute_value_mlt": [{"subitem_publisher": "Frontiers Media SA"}, {"subitem_publisher": "鹿児島大学"}]}, "item_5_subject_15": {"attribute_name": "NDC", "attribute_value_mlt": [{"subitem_subject": "490", "subitem_subject_scheme": "NDC"}]}, "item_5_text_24": {"attribute_name": "公開者よみ", "attribute_value_mlt": [{"subitem_text_value": "カゴシマ ダイガク"}]}, "item_5_text_25": {"attribute_name": "公開者別名", "attribute_value_mlt": [{"subitem_text_value": "Kagoshima University"}]}, "item_5_text_44": {"attribute_name": "備考", "attribute_value_mlt": [{"subitem_text_value": "【指導教員:岡本康裕】"}]}, "item_5_text_47": {"attribute_name": "date.appl", "attribute_value_mlt": [{"subitem_text_value": "【学位申請日】2022-11-02"}]}, "item_5_version_type_14": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": 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"item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Escherichia albertii", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "flagellin", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "fliC", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "H-antigen", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "genotyping", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Diversification of Escherichia albertii H-antigens and Development of H-Genotyping PCR", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Diversification of Escherichia albertii H-antigens and Development of H-Genotyping PCR"}]}, "item_type_id": "5", "owner": "29", "path": ["86"], "permalink_uri": "http://hdl.handle.net/10232/00032424", "pubdate": {"attribute_name": "公開日", "attribute_value": "2023-03-14"}, "publish_date": "2023-03-14", "publish_status": "0", "recid": "16540", "relation": {}, "relation_version_is_last": true, "title": ["Diversification of Escherichia albertii H-antigens and Development of H-Genotyping PCR"], "weko_shared_id": -1}