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The ArcAB two-component system is associated with the susceptibility of Aggregatibacter actinomycetemcomitans to superoxide and hydrogen peroxide
http://hdl.handle.net/10232/0002001673
http://hdl.handle.net/10232/000200167394fb92c4-b73d-4dac-a298-1d65f21dd543
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| アイテムタイプ | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2025-11-06 | |||||
| タイトル | ||||||
| タイトル | The ArcAB two-component system is associated with the susceptibility of Aggregatibacter actinomycetemcomitans to superoxide and hydrogen peroxide | |||||
| タイトル言語 | en | |||||
| 著者 |
RATMAN FARID MOHAMMAD
× RATMAN FARID MOHAMMAD |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | ArcAB | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Aggregatibacter actinomycetemcomitans | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | superoxide dismutase | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Aggregatibacter actinomycetemcomitans is a Gram-negative facultative anaerobe and is associated with periodontal disease. This bacterium is exposed to environmental stresses, such as osmotic pressure, temperature shifts, pH shifts, and antimicrobial substances, including reactive oxygen species (ROS), in the human oral cavity. The bacterial two-component system ArcAB modulates gene expression in response to environmental changes, primarily by sensing oxygen pressure in several pathogens belonging to the γ-proteobacteria. It is also known to provide adaptation to ROS stress; however, its function in A. actinomycetemcomitans remains unclear. In this study, we found that the expression of sod, which encodes superoxide dismutase, was increased in the inactivated mutant of arcA, which encodes a response regulator. The mutant exhibited reduced susceptibility to superoxide and hydrogen peroxide (H2O2). Additionally, this strain showed reduced susceptibility to H2O2 from Streptococcus sanguinis and increased survival in macrophages. Since ArcB is the cognate histidine kinase of ArcA, the inactivated mutant of arcB was analyzed for its phenotypes. The arcB mutant exhibited reduced susceptibility to superoxide and H2O2. Compared to wild type, the phosphorylation level of ArcA in the arcB mutant was decreased. These results suggest that the ArcA response regulator receives phosphate groups from ArcB histidine kinase and negatively regulates the expression of sod, thereby affecting bacterial survival in response to ROS produced by oral commensals and host immune cells. | |||||
| 内容記述言語 | en | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Ratman MF, Oogai Y, Matsumoto A, Nakata M The ArcAB two-component system is associated with the susceptibility of Aggregatibacter actinomycetemcomitans to superoxide and hydrogen peroxide mSphere10:e00019-25.2025. https://doi.org/10.1128/msphere.00019-25 |
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| 内容記述言語 | en | |||||
| 作成日 | ||||||
| 日付 | 2025-08-08 | |||||
| 日付タイプ | Collected | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| ファイル（説明） | ||||||
| 内容記述 | 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨 | |||||
| 公開者・出版者 | ||||||
| 出版者 | American Society for Microbiology | |||||
| 出版者言語 | en | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 備考 | ||||||
| 備考言語 | ja | |||||
| 備考 | 【指導教員:西條英人】 | |||||
| date.appl | ||||||
| date.appl | 【学位申請日】2025-07-02 | |||||
| 学位名 | ||||||
| 学位名 | 博士(歯学) | |||||
| 学位名の言語 | ja | |||||
| 学位名 | ||||||
| 学位名 | Doctor of Philosophy in Dental Science | |||||
| 学位名の言語 | en | |||||
| 学位授与機関名 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 17701 | |||||
| 学位授与機関名 | 鹿児島大学 | |||||
| 学位授与機関名の言語 | ja | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2025-08-29 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲総研第797号 | |||||
