{"_buckets": {"deposit": "0c87ea3a-c53e-4a38-9b2a-15cd4f4124f1"}, "_deposit": {"created_by": 3, "id": "3064", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "3064"}, "status": "published"}, "_oai": {"id": "oai:ir.kagoshima-u.ac.jp:00003064", "sets": ["81"]}, "author_link": ["15498", "96436", "96437"], "item_8_alternative_title_18": {"attribute_name": "別言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Solubilization-inducing tag for solubilization, development of higher function and medicine for proteins."}]}, "item_8_alternative_title_19": {"attribute_name": "タイトルよみ", "attribute_value_mlt": [{"subitem_alternative_title": "タンパクシツ ノ コウカヨウカ・ コウキノウカ・ チリョウヤク カイハツ オ メザス 「カヨウカ ユウドウ コウエンセイ タグ」 ノ ソウセイホウ"}]}, "item_8_date_6": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2014-06-11"}]}, "item_8_description_4": {"attribute_name": "要約(Abstract)", "attribute_value_mlt": [{"subitem_description": "2011-2013年度科学研究費助成事業（基盤研究（C））研究成果報告書　課題番号：23580475　研究代表者：徳永正雄（鹿児島大学・農学部・教授）", "subitem_description_type": "Other"}, {"subitem_description": "好塩性酵素は、総荷電が大きくマイナスに偏った酸性蛋白質であり、広いpH領域で多量のマイナス荷電をもち、通常酵素が機能しない高濃度塩存在下でも「塩析効果」を受けず機能できる。高次構造を壊す他のストレス環境下でも多量のマイナス荷電による高可溶性と荷電の反発による「非凝集性」を保持し、可逆的な構造回復能を示す「不可逆的に変性しない酵素」である。この好塩性タンパク質遺伝子を分離、大量発現させ、性質検討を行った。また、融合タンパク質作成のタグとしてその全長または部分配列を利用した。", "subitem_description_type": "Other"}, {"subitem_description": "Halophilic proteins, acidic proteins with high content of negative charge, can function under high salt conditions without salting-out effects of salts. High solubility and negative charge repulsion make halophilic protein resistant to various stresses which induce denaturaion of protein, and thus, holophilic proteins are highly resistant to denaturation (resistant to irreversible-aggregation). We isolated several genes of halophilic proteins, characterized, and developed tag to construct fusion proteins.", "subitem_description_type": "Other"}]}, "item_8_full_name_2": {"attribute_name": "著者よみ", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "96436", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "トクナガ, マサオ"}]}]}, "item_8_full_name_3": {"attribute_name": "別言語の著者", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "96437", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "TOKUNAGA, Masao"}]}]}, "item_8_publisher_23": {"attribute_name": "公開者・出版者", "attribute_value_mlt": [{"subitem_publisher": "鹿児島大学"}]}, "item_8_subject_15": {"attribute_name": "NDC", "attribute_value_mlt": [{"subitem_subject": "491", "subitem_subject_scheme": "NDC"}]}, "item_8_text_24": {"attribute_name": "公開者よみ", "attribute_value_mlt": [{"subitem_text_value": "カゴシマ ダイガク"}]}, "item_8_text_25": {"attribute_name": "公開者別名", "attribute_value_mlt": [{"subitem_text_value": "Kagoshima University"}]}, "item_8_text_30": {"attribute_name": "NIIタイプ", "attribute_value_mlt": [{"subitem_text_value": "Research Paper"}]}, "item_8_text_41": {"attribute_name": "科研費番号 ", "attribute_value_mlt": [{"subitem_text_value": "23590547"}]}, "item_8_version_type_14": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "徳永, 正雄"}], "nameIdentifiers": [{"nameIdentifier": "15498", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000020112782", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-10-27"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "23580475.pdf", "filesize": [{"value": "423.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 423400.0, "url": {"label": "23580475.pdf", "url": "https://ir.kagoshima-u.ac.jp/record/3064/files/23580475.pdf"}, "version_id": "470d98b9-3242-40b6-933d-4f35018917c2"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "好塩性蛋白質", "subitem_subject_scheme": "Other"}, {"subitem_subject": "可溶化", "subitem_subject_scheme": "Other"}, {"subitem_subject": "高次構造形成", "subitem_subject_scheme": "Other"}, {"subitem_subject": "まき戻り", "subitem_subject_scheme": "Other"}, {"subitem_subject": "凝集耐性", "subitem_subject_scheme": "Other"}, {"subitem_subject": "タグ", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "タンパク質の高可溶化・高機能化・治療薬開発をめざす「可溶化誘導好塩性タグ」の創製法", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "タンパク質の高可溶化・高機能化・治療薬開発をめざす「可溶化誘導好塩性タグ」の創製法"}]}, "item_type_id": "8", "owner": "3", "path": ["81"], "permalink_uri": "http://hdl.handle.net/10232/24165", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-05-22"}, "publish_date": "2015-05-22", "publish_status": "0", "recid": "3064", "relation": {}, "relation_version_is_last": true, "title": ["タンパク質の高可溶化・高機能化・治療薬開発をめざす「可溶化誘導好塩性タグ」の創製法"], "weko_shared_id": 3}