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連続切片の in situ ハイブリダイゼーションを利用した遺伝子発現の3次元再構築法
http://hdl.handle.net/10232/26731
http://hdl.handle.net/10232/26731a576944b-e634-4227-baea-fcada8bb6dbb
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-05-13 | |||||
タイトル | ||||||
タイトル | 連続切片の in situ ハイブリダイゼーションを利用した遺伝子発現の3次元再構築法 | |||||
タイトルよみ | ||||||
タイトルよみ | レンゾク セッペン ノ in situ ハイブリダイゼーション オ リヨウシタ イデンシ ハツゲン ノ 3ジゲン サイコウチクホウ | |||||
著者 |
山中, 淳之
× 山中, 淳之× 岩井, 治樹× 倉本, 恵梨子× 後藤, 哲哉 |
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著者よみ | ||||||
姓名 | ヤマナカ, アツシ | |||||
著者よみ | ||||||
姓名 | イワイ, ハルキ | |||||
著者よみ | ||||||
姓名 | クラモト, エリコ | |||||
著者よみ | ||||||
姓名 | ゴトウ, テツヤ | |||||
別言語の著者 | ||||||
姓名 | YAMANAKA, Atsushi | |||||
別言語の著者 | ||||||
姓名 | IWAI, Haruki | |||||
別言語の著者 | ||||||
姓名 | KURAMOTO, Eriko | |||||
別言語の著者 | ||||||
姓名 | GOTO, Tetsuya | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 3-D reconstruction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | in situ hybridization | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | serial histological sections | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | gene expression | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tooth development | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
要約(Abstract) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Identifying the cells expressing a specific gene in adult or developing tissues is the most basic step in the field of histochemistry and developmental biology. This article presents a sophisticated method of visualizing three-dimensional (3-D) gene expression patterns in organs or tissues with high precision. In this method, 3-D gene expression data are reconstructed on a computer from serial histological sections after in situ hybridization is performed. As an application of this method, we reconstructed 3-D gene expression patterns in the developing tooth, which is an organ with complex morphology, such as a number of cusps on the tooth crown. This method showed several advantages over the usual procedures, enabling us to extract any tissue structures and compare them with 3-D expression patterns of specific genes. In addition, it enabled us to reconstruct 3-D complex tissue morphology and gene expression patterns of the developing tooth in great detail and examine their spatial relationship with accuracy. Thus, the present method will be applicable to various organs and tissues, and can offer more precise and useful information about gene expression patterns in the field. | |||||
収録雑誌名 |
鹿児島大学歯学部紀要 巻 35, p. 87-92, 発行日 2015 |
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作成日 | ||||||
日付 | 2015-03-25 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
ISSN | 0389-7834 | |||||
NII書誌ID(雑誌) | ||||||
収録物識別子タイプ | NCID | |||||
NC ID | AN0035442X | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
NDC | ||||||
主題Scheme | NDC | |||||
主題 | 464.1 | |||||
公開者・出版者 | ||||||
出版者 | 鹿児島大学 | |||||
公開者よみ | ||||||
公開者よみ | カゴシマ ダイガク | |||||
公開者別名 | ||||||
公開者別名 | Kagoshima University |