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ムラミルペプチドによるモルモットマクロファージの活性化と分化に関する研究
http://hdl.handle.net/10232/355
http://hdl.handle.net/10232/35549a8c3d9-dfd4-4fda-9510-ea0c95f0be78
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000165789.pdf (3.8 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2015-04-30 | |||||||||
| タイトル | ||||||||||
| タイトル | ムラミルペプチドによるモルモットマクロファージの活性化と分化に関する研究 | |||||||||
| タイトル言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Activation and Differentiation of Guinea pig Macrophage by Muramy Dipeptide | |||||||||
| タイトル言語 | en | |||||||||
| 著者 |
永尾, 重喜
× 永尾, 重喜
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| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Bacterial cell wall peptidoglycan | |||||||||
| キーワード | ||||||||||
| 主題言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Muramyl dipeptide | |||||||||
| キーワード | ||||||||||
| 主題言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Adijuvant activity | |||||||||
| キーワード | ||||||||||
| 主題言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Macrophage activation | |||||||||
| キーワード | ||||||||||
| 主題言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Epithelioid granuloma | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 要約 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | Muramyl dipeptide (MDP) is a minimal structure necessory for various biological activities of bacterial cell wall peptidoglycans. MDP induces in guinea pigs the granulomas containing macrophages and epithelioid cells differentiated from macrophages. This suggests that MDP differentiates macrophages into epithelioid cells. This study was undertaken to gain an insight into the mechanism of activation and differentiation of macrophage induced by MDP. For this purpose, the effect of MDP on macrophage DNA synthesis was studied in view of the generally accepted fact that DNA synthesis is decreased when cells differentiate. Guinea pig peritoneal exudate macrophages actively incorporated ^3H-thymidine into trichloroacetic acidin soluble fraction in vitro without macrophage proliferating factors. The incorporation of ^3H-Thymidine was almost completely inhibited by aphidicolin, an inhibitor of DNA polymerase-α and an autoradiograph showed heavy labeling in nuclei of about 200% of macrophage populations. These results indicate that the observed thymidine incorporation reflected a DNA synthesis for replication. When macrophage was stimulated by MDP, the ^3H-Thymidine incorporation was markedly suppressed while ^<14>C-glucosamine incorporation and production of monokine were increased. The suppression of ^3H-thymidine incorporation by MDP was not due to the decrease in thymidine transport through the cell membrane. An autoradiograph revealed that MDP decreased markedly the number of macrophages whose nuclei were labeled by ^3H-Thymidine. These resuts indicate that suppresion of ^3H-thymidine incorporation by MDP was due to a true inhibition of DNA synthesis. Among the three tested enzymes involved in DNA synthesis, only thymidine kinase activity decreased progressively in parallel with the decline in ^3H-Thymidine incorporation. Cyclic AMP and PGE_2,both of which are increased in amount in macrophage by MDP stimulation, suppressed DNA synthesis of macrophage. Phorbol myristic acid (PMA), ionomycin and debutyryl cyclic GMP failed to affect the ^3H-Thymidine incorporation. Inhibitors of cyclic AMP-dependent protein kinase considerably reduced the suppressive effect on ^3H-Thymidine incorporation. These results suggest that cyclic AMP or cyclic AMP-dependent protein kinase might be play an important role in suppression of ^3H-Thymidine incorporation. Concerning various analogs of MDP, a close correlation was observed among the capacities of activating macrophages, suppressing DNA synthesis and inducing epithelioid granuloma formation. These findings suggest that suppression of DNA synthesis by MDP may be induced by macrophage activation and represent an initial event during the differentiation of macrophage for epithelioid cells. | |||||||||
| 内容記述言語 | en | |||||||||
| 収録雑誌名 |
ja : 鹿児島大学歯学部紀要 en : Annals of Kagoshima University Dental School 巻 15, p. 15-36, 発行日 1995-03-25 |
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| 作成日 | ||||||||||
| 日付 | 1995-03-25 | |||||||||
| 日付タイプ | Issued | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| ISSN | 03897834 | |||||||||
| NII書誌ID（雑誌） | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| NC ID | AN0035442X | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| NDC | ||||||||||
| 主題Scheme | NDC | |||||||||
| 主題 | 497 | |||||||||
| NIIsubject | ||||||||||
| 主題言語 | ja | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | 歯学 | |||||||||
| 公開者・出版者 | ||||||||||
| 出版者 | 鹿児島大学 | |||||||||
| 出版者言語 | ja | |||||||||
| 公開者・出版者 | ||||||||||
| 出版者 | Kagoshima University | |||||||||
| 出版者言語 | en | |||||||||
