{"_buckets": {"deposit": "526dd1cd-95b5-4206-9d77-242e2164f69b"}, "_deposit": {"created_by": 3, "id": "7891", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "7891"}, "status": "published"}, "_oai": {"id": "oai:ir.kagoshima-u.ac.jp:00007891", "sets": ["40", "4820"]}, "author_link": ["103770", "103771", "103760", "103769", "103772", "103763", "103762", "103761", "103764"], "item_7_alternative_title_18": {"attribute_name": "別言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "ウナギ肝臓から限定分解したグルタミン酸脱水素酵素の精製"}]}, "item_7_biblio_info_5": {"attribute_name": "収録雑誌名", "attribute_value_mlt": [{"bibliographicPageEnd": "17", "bibliographicPageStart": "11", "bibliographicVolumeNumber": "37", "bibliographic_titles": [{"bibliographic_title": "鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University"}]}]}, "item_7_date_6": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "1988-12-25"}]}, "item_7_description_4": {"attribute_name": "要約(Abstract)", "attribute_value_mlt": [{"subitem_description": "Glutamate dehydrogenase (GDH) suffered limited proteolysis was purified from the acetone\npowder of the eel liver. Limited proteolysis of GDH occurred at heat treatment in purification\nprocedures, but it was inhibited by leupeptin, an inhibitor of trypsin or thiol proteases.\nFurthermore, GDH suffered limited proteolysis during preservation of the liver at -20°C, but\nGDH purified from preserved livers, which was perfused with phosphate buffered saline\ncontaining leupeptin preliminarily, was almost native form. Molecular weight and pI of limited\nproteolyzed GDH were 340,000 and pH 5.9, respectively. There was no difference in molecular\nweight and isoelectric point between native and limited proteolyzed GDHs in oligomer state.\nHowever, subunit of GDH suffered limited proteolysis consisted of two kinds of proteins with\ndifferent molecular weight. One was 53,000 to 55,000 dalton and the other 50,000 to 52,000\ndalton. Native GDH consisted of only the former proteins with 53,000 to 55,000 dalton.\np-Chloromercuribenzoic acid inhibited the activity of GDH suffered limited proteolysis, but\nstimulated that of native GDH. The effect of allosteric effectors, ADP and GTP, on native\nGDH was more drastic than that on GDH suffered limited proteolysis.\nHypothesis for physiological meaning of GDH suffered limited proteolysis was also\ndiscussed.", "subitem_description_type": "Other"}]}, "item_7_full_name_3": {"attribute_name": "別言語の著者", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "103769", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "林, 征一"}]}, {"nameIdentifiers": [{"nameIdentifier": "103770", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "福山, 恵子"}]}, {"nameIdentifiers": [{"nameIdentifier": "103771", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "湯, 敏謙"}]}, {"nameIdentifiers": [{"nameIdentifier": "103772", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "大城, 善太郎"}]}]}, "item_7_publisher_23": {"attribute_name": "公開者・出版者", "attribute_value_mlt": [{"subitem_publisher": "鹿児島大学"}]}, "item_7_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0453087X", "subitem_source_identifier_type": "ISSN"}]}, "item_7_source_id_9": {"attribute_name": "NII書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00040498", "subitem_source_identifier_type": "NCID"}]}, "item_7_subject_15": {"attribute_name": "NDC", "attribute_value_mlt": [{"subitem_subject": "660", "subitem_subject_scheme": "NDC"}]}, "item_7_text_24": {"attribute_name": "公開者よみ", "attribute_value_mlt": [{"subitem_text_value": "カゴシマ ダイガク"}]}, "item_7_text_25": {"attribute_name": "公開者別名", "attribute_value_mlt": [{"subitem_text_value": "Kagoshima University"}]}, "item_7_text_30": {"attribute_name": "NIIタイプ", "attribute_value_mlt": [{"subitem_text_value": "Departmental Bulletin Paper"}]}, "item_7_version_type_14": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "HAYASHI, Seiichi"}], "nameIdentifiers": [{"nameIdentifier": "103760", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "FUKUYAMA, Keiko"}], "nameIdentifiers": [{"nameIdentifier": "103761", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TANG, Min Qian"}], "nameIdentifiers": [{"nameIdentifier": "103762", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MORSHED, Monzoor"}], "nameIdentifiers": [{"nameIdentifier": "103763", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "OOSHIRO, Zentaro"}], "nameIdentifiers": [{"nameIdentifier": "103764", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-10-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AN00040498_v37_p11-17.pdf", "filesize": [{"value": "574.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 574600.0, "url": {"label": "AN00040498_v37_p11-17.pdf", "url": "https://ir.kagoshima-u.ac.jp/record/7891/files/AN00040498_v37_p11-17.pdf"}, "version_id": "ccc6fa87-e4a9-4327-852f-576150113f4d"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Glutamate dehydrogenase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Liver", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Eel", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Proteolyzed GDH", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Limited Proteolysis", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Purification of Eel Liver Glutamate Dehydrogenase Suffered Limited Proteolysis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Purification of Eel Liver Glutamate Dehydrogenase Suffered Limited Proteolysis"}]}, "item_type_id": "7", "owner": "3", "path": ["40", "4820"], "permalink_uri": "http://hdl.handle.net/10232/13366", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-05-07"}, "publish_date": "2012-05-07", "publish_status": "0", "recid": "7891", "relation": {}, "relation_version_is_last": true, "title": ["Purification of Eel Liver Glutamate Dehydrogenase Suffered Limited Proteolysis"], "weko_shared_id": 3}