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ウナギ肝臓から限定分解したグルタミン酸脱水素酵素の精製
http://hdl.handle.net/10232/13366
http://hdl.handle.net/10232/133667d9b350a-15d7-4b64-8c6e-32e7e2e4ca9a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00040498_v37_p11-17.pdf (574.6 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-05-07 | |||||
| タイトル | ||||||
| タイトル | Purification of Eel Liver Glutamate Dehydrogenase Suffered Limited Proteolysis | |||||
| タイトル言語 | en | |||||
| タイトル | ||||||
| タイトル | ウナギ肝臓から限定分解したグルタミン酸脱水素酵素の精製 | |||||
| タイトル言語 | ja | |||||
| 著者 |
林, 征一
× 林, 征一× 福山, 恵子× 湯, 敏謙× MORSHED, Monzoor× 大城, 善太郎 |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Glutamate dehydrogenase | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Liver | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Eel | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Proteolyzed GDH | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Limited Proteolysis | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Glutamate dehydrogenase (GDH) suffered limited proteolysis was purified from the acetone powder of the eel liver. Limited proteolysis of GDH occurred at heat treatment in purification procedures, but it was inhibited by leupeptin, an inhibitor of trypsin or thiol proteases. Furthermore, GDH suffered limited proteolysis during preservation of the liver at -20°C, but GDH purified from preserved livers, which was perfused with phosphate buffered saline containing leupeptin preliminarily, was almost native form. Molecular weight and pI of limited proteolyzed GDH were 340,000 and pH 5.9, respectively. There was no difference in molecular weight and isoelectric point between native and limited proteolyzed GDHs in oligomer state. However, subunit of GDH suffered limited proteolysis consisted of two kinds of proteins with different molecular weight. One was 53,000 to 55,000 dalton and the other 50,000 to 52,000 dalton. Native GDH consisted of only the former proteins with 53,000 to 55,000 dalton. p-Chloromercuribenzoic acid inhibited the activity of GDH suffered limited proteolysis, but stimulated that of native GDH. The effect of allosteric effectors, ADP and GTP, on native GDH was more drastic than that on GDH suffered limited proteolysis. Hypothesis for physiological meaning of GDH suffered limited proteolysis was also discussed. |
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| 内容記述言語 | en | |||||
| 収録雑誌名 |
ja : 鹿児島大学水産学部紀要 en : Memoirs of Faculty of Fisheries Kagoshima University 巻 37, p. 11-17, 発行日 1988-12-25 |
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| 作成日 | ||||||
| 日付 | 1988-12-25 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| ISSN | 0453087X | |||||
| NII書誌ID（雑誌） | ||||||
| 収録物識別子タイプ | NCID | |||||
| NC ID | AN00040498 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 660 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 出版者言語 | en | |||||
