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好酸球性副鼻腔炎における好酸球活性化機序の解明
http://hdl.handle.net/10232/14793
http://hdl.handle.net/10232/1479370defb25-f734-4958-887f-9a7d80d41c35
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
22791613seika.pdf (191.5 kB)
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| Item type | 研究報告書 / Research Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-12-24 | |||||
| タイトル | ||||||
| タイトル | 好酸球性副鼻腔炎における好酸球活性化機序の解明 | |||||
| タイトル言語 | ja | |||||
| タイトル | ||||||
| タイトル | Elucidation of the mechanism of eosinophil activation in eosinophilic sinusitis | |||||
| タイトル言語 | en | |||||
| 著者 |
吉福, 孝介
× 吉福, 孝介 |
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| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題言語 | ja | |||||
| 主題Scheme | Other | |||||
| 主題 | 好酸球性副鼻腔炎 | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | VEGF | |||||
| キーワード | ||||||
| 主題言語 | ja | |||||
| 主題Scheme | Other | |||||
| 主題 | デキサメタゾン | |||||
| キーワード | ||||||
| 主題言語 | ja | |||||
| 主題Scheme | Other | |||||
| 主題 | 鼻茸線維芽細胞 | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | eotaxin | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
| 資源タイプ | research report | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2010-2011年度科学研究費助成事業(科学研究費補助金(若手研究(B)))研究成果報告書 課題番号：22791613 研究代表者：吉福孝介 (鹿児島大学医学部・歯学部附属病院講師) | |||||
| 内容記述言語 | ja | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 好酸球浸潤の多い鼻茸群では好酸球浸潤の少ない鼻茸群と比較して、LPS刺激による線維芽細胞のVEGF 産生は有意に上昇していた。デキサメタゾン（10^{-6}mol）によりVEGF産生は有意に抑制された。VEGFの免疫染色において、線維芽細胞は好酸球浸潤の多い鼻茸群では有意に強く染まっていた。好酸球浸潤の多い鼻茸群では好酸球浸潤の少ない鼻茸群と比較してVEGF産生をする線維芽細胞数が多く、またLPS刺激などの外因因子が作用することでVEGF産生がより高い結果であった。VEGF産生が高いことは鼻茸の発生の一因である可能性があると思われた。 | |||||
| 内容記述言語 | ja | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The mechanism by which eosinophils are selectively recruited in nasal polyp remains to be clarified.The exact mechanisms by which glucocorticoids exert their beneficial effect on nasal polyp remain to be clearly defined In the present study,the secreted levels of VEGF from the culured fibroblasts and VEGF in the nasal secretion were determined. The level were compared between Enp and NEnp and the role of those chemokines in the pathogenesis of nasal polyp is discussed. Tissues from nasal polyps obtained by biopsy was fixed in formarin and stained with hematoxylin and eosin, and the number of eosinophils was counted at ×400 magnification under light-microscopy. Three fields were examined for each section and the average was considered the number of eosinophils infiltrating the sample. Clinical features of Enp are complication with Asthma, bilateral nasal polyps and Ethmoidal sinus disease are often recognized in CT. These three items were examined, statistically significant difference in 40-60. So we classified samples having more than 60 eosinophils as Enp, having less than 40 eosinophils as Nenp in this study. 17 CRS with nasal polyp cases were included in the Nasal leakage study and 11 CRS with nasal polyp cases were included in the cultured fibroblast study. None of the subjects had taken oral steroids for more than year before the biopsy. Fibroblasts isolated from nasal polyps were cultured. After dexamethasone(DEX) pretreatment,stimulated with 10ng/ml of tumor necrosis factor-α(TNF-α)and interleukin-4(IL-4)for 24 hours or stimulated with 10μg/ml of LPS. After stimulation, culture supernatants were collected and concentration of VEGF were quantified by Enzyme linked immunosorbent assay(ELISA). Nasal secretions from nasal cavity were collected by JUHN TYM-TUP(Medtronic Xomed,Inc,Jacksonville,FL). Collected nasal secretions were diluted with 2 ml phosphate-buffered saline(PBS) and centrifuged at 350 ×g for 10 minutes. The supernatant was then harvested. VEGF levels in the supernatants were measured by ELISA. VEGF were significantly lower in the DEX10^{-6}DEX treated group compared to non-DEXtreated group. Conclusions: VEGF plays an important role in the selective recruitment of eosinophils in Enp. Dex may inhibit nasal polyp growth local reguration of decreased VEGF. | |||||
| 内容記述言語 | en | |||||
| 作成日 | ||||||
| 日付 | 2012-05-15 | |||||
| 日付タイプ | Collected | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 496 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 出版者言語 | en | |||||
| 科研費番号 | ||||||
| 科研費番号 | 22791613 | |||||
