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沖縄産亜熱帯植物から分離したフラボノイド配糖体の化学特性とそれらの生理活性に関する研究
http://hdl.handle.net/10232/15157
http://hdl.handle.net/10232/15157a881e819-fd04-4450-b8de-2f31889bba24
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renken668.pdf (309.7 kB)
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Diss_renken668_Leong Cheng Ning Abraham_20100315.pdf (1.1 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2015-02-19 | |||||
| タイトル | ||||||
| タイトル | Studies on the chemical characteristics of flavonoidglycosides isolated from subtropical plants in Okinawa and their biological activities | |||||
| タイトル言語 | en | |||||
| タイトル | ||||||
| タイトル | 沖縄産亜熱帯植物から分離したフラボノイド配糖体の化学特性とそれらの生理活性に関する研究 | |||||
| タイトル言語 | ja | |||||
| 著者 |
リョン, チェン ニン アブラハム
× リョン, チェン ニン アブラハム |
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| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 連合農学研究科博士論文(農学) : 学位取得日: 平成22年3月15日 | |||||
| 内容記述言語 | ja | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Free radicals and reactive oxygen species do not only cause denaturation in foods but also cause oxidative damage to the human body. The development of diabetic complications was shown to be related with the excessive oxidation by these molecular species and hyperglycemia, which produced reactive carbonyl species (RCS) and glycation of protein. Natural antioxidants from plants such as flavonoids and polyphenols were found to reduce oxidative damage to food during storage and protecting the human body cells from oxidative damage. Okinawa plants are reported to contain richly in antioxidants, which might be contributed to the development of good health to the Okinawa people. Two Okinawa plants, Ooitabi (Ficus pumila L.) and Taumu (Colocasia esculenta S.) were selected in this research. The antioxidant components in these plants were investigated and their structuralactivity relationship was further evaluated. One of the isolated antioxidant compounds from Ooitabi, rutin, was also investigated for its potential in inhibiting the formation of RCS from glucose autoxidation and glycation of an amine under high glucose concentration. The separation of antioxidants from Ooitabi and Taumu were based on their DPPH radical scavenging activities. Ooitabi and Taumu were extracted with aqueous ethanol and methanol, respectively. The extracts of these plants were fractionated using C18 solid phase extraction followed by a series of separation using reversed phase MPLC and HPLC. All isolated compounds were identified with MS and NMR spectroscopies. Four flavonoid glycosides had been isolated and identified from Ooitabi, which were apigenin 6neohesperidosyl, kaempferol 3robinobioside, kaempferol 3rutinoside and rutin. As for Taumu, seven flavonoid glycosides were also isolated and identified as isoorientin, isoschaftoside, isovitexin, luteolin 7sophoroside, orientin, schaftoside and vitexin. The structuralactivity relationship of these flavonoid glycosides were evaluated with comparison to apigenin, isoquercitrin, kaempferol, luteolin, quercetin and quercitrin using DPPH radical scavenging, superoxide radical inhibitory and bcarotene bleaching assays. It was found that not only the presence of hydroxyl groups in position 3 and 3' of the aglycone had an effect on the antioxidant activities but also the glycosylation characteristics such as the glycosidic bonding position, bonding type, the number of glycosides and the type of glycosides. Analysis on the ranking of the antioxidant activities of these compounds in these three assays showed that they have different mechanisms of actions. Hyperglycemic is an important characteristic of diabetes mellitus. The formation of RCS was found to increase under high sugar concentration and high temperature. The presence of air and metal ions such as Cu2+ and Fe2+ were found to promote its formation. RCS was found to react readily with lysine to form intermediate advanced glycation end products (AGEs), one of the contributing factors of diabetic complications. Rutin as one of the strong flavonoid antioxidant was shown to inhibit the formation of RCS as well as the glycation of lysine under high glucose concentration. The results of this study suggested that the flavonoid glycosides in Ooitabi and Taumu as antioxidants have potential in inhibiting the formation of intermediate AGEs. Hence, these Okinawa plants can be used as a part of dietary management for diabetes patients and health promotion. |
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| 内容記述言語 | en | |||||
| 作成日 | ||||||
| 日付 | 2010-03-15 | |||||
| 日付タイプ | Issued | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 498 | |||||
| ファイル（説明） | ||||||
| 内容記述 | 学位論文の要旨, 学位論文全文 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | カゴシマ ダイガク | |||||
| 出版者言語 | ja-Kana | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 出版者言語 | en | |||||
| 備考 | ||||||
| 備考 | 指導教員: 田幸正邦(琉球大学) | |||||
| 学位記番号 | ||||||
| 学位記番号 | 連研第668号 | |||||
| 学位名 | ||||||
| 学位名の言語 | ja | |||||
| 学位名 | 博士(農学) | |||||
| 学位名 | ||||||
| 学位名の言語 | en | |||||
| 学位名 | Doctor of Philosophy in Agricultural Science | |||||
| 学位授与機関名 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 17701 | |||||
| 学位授与機関名の言語 | ja | |||||
| 学位授与機関名 | 鹿児島大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2010-03-15 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲連研第668号 | |||||
