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Fluorescent Staining Analysis of Chromosomes in Pear (Pyrus spp.)
http://hdl.handle.net/10232/18906
http://hdl.handle.net/10232/18906d19dc397-171b-42a7-8716-374c1195164a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2013-07-29 | |||||
| タイトル | ||||||
| タイトル | Fluorescent Staining Analysis of Chromosomes in Pear (Pyrus spp.) | |||||
| タイトル言語 | en | |||||
| 著者 |
山本, 雅史
× 山本, 雅史× 高田, 教臣× 平林, 利郎× 久保, 達也× 冨永, 茂人 |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | chromomycin A3 | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | CMA | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | DAPI | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | karyotype | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | PI | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | propidium iodide | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Fluorescent banding patterns of pear chromosomes were determined from samples taken from root tips of openpollinated seedlings of six cultivars from three species [Pyrus pyrifolia (Burm. F) Nakai (Japanese pear), P. communis L. (European pear), and P. bretschneideri Rehder (Chinese pear)]. Root tips were pretreated in 2mM 8-hydroxyquinoline at 10°C for 4 h; the chromosome samples were prepared by the enzymatic maceration and air-drying method. All cultivars used in this study had 2n = 34 chromosomes. Chromomycin A3 (CMA) positive (+) bands were observed in telomeric positions of four chromosomes. In some samples, these CMA + bands were observed at satellite positions. 4'-6-diamidino-2-phenylindole (DAPI) negative bands (−) were seen to correspond with CMA + bands. Thirty chromosomes had no CMA +/DAPI − bands. No propidium iodide (PI) bands were observed in any chromosomes. The number and positions of CMA + bands were stable, and there was no difference among the three species. |
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| 内容記述言語 | en | |||||
| 収録雑誌名 |
en : Journal of the Japanese Society for Horticultural Science 巻 79, 号 1, p. 23-26, 発行日 2010 |
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| 作成日 | ||||||
| 日付 | 2010-01-01 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| ISSN | 18823351 | |||||
| NII書誌ID(雑誌) | ||||||
| 収録物識別子タイプ | NCID | |||||
| NC ID | AA12177046 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| DOI | https://doi.org/10.2503/jjshs1.79.23 | |||||
| 権利 | ||||||
| 権利情報の言語 | ja | |||||
| 権利情報 | 園芸学会 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 625 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 園芸学会 | |||||
| 出版者言語 | ja | |||||