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Fluorescent Staining and Fluorescence in situ Hybridization of rDNA of Chromosomes in Pear (Pyrus spp.)
http://hdl.handle.net/10232/18909
http://hdl.handle.net/10232/189092f1edc75-b705-4660-9b74-9e28c9c2c5ee
名前 / ファイル | ライセンス | アクション |
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YAMAMOTO_J-Japan-Soc-Hort-Sci_v81n1.pdf (470.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-07-29 | |||||
タイトル | ||||||
タイトル言語 | en | |||||
タイトル | Fluorescent Staining and Fluorescence in situ Hybridization of rDNA of Chromosomes in Pear (Pyrus spp.) | |||||
著者 |
山本, 雅史
× 山本, 雅史× 高田, 教臣× 山本, 俊哉× 寺上, 伸吾× 滋田, 徳美× 久保, 達也× 冨永, 茂人 |
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言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | CMA | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | DAPI | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | FISH | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | McFISH | |||||
キーワード | ||||||
主題言語 | en | |||||
主題Scheme | Other | |||||
主題 | ribosomal RNA gene | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
要約(Abstract) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Fluorescent banding patterns of pear chromosomes were determined from samples taken from root tips of open-pollinated seedlings of Pyrus pyrifolia (Burm. F) Nakai (Japanese pear), P. calleryana Decne. (Callery pear), P. pyrifolia × P. ussuriensis var. aromatica (a hybrid of Japanese pear and Iwateyamanashi), and P. mikawana Koidz. (Toyotomi Nashi). All accessions used in this study had 2n = 34 chromosomes. Chromomycin A3 (CMA)-positive (+) bands were observed in telomeric positions of four chromosomes in all accessions. 4'-6-diamidino-2-phenylindole (DAPI)-negative bands (−) corresponded with CMA+ bands. Fluorescence in situ hybridization (FISH) was conducted using open-pollinated seedlings of ‘Osa Gold’ (Pyrus pyrifolia) and Toyotomi Nashi (P. mikawana) as materials. Four out of six 18S-5.8S-25S ribosomal RNA gene (rDNA) sites corresponded with CMA+/DAPI− bands. The 5S rDNA sites were detected in centromeric positions of two chromosomes. Two centromeric 5S rDNA and six telomeric 18S-5.8S-25S rDNA sites were located on different chromosomes as determined from the results of multi-color FISH. | |||||
内容記述言語 | en | |||||
要約(Abstract) | ||||||
内容記述タイプ | Other | |||||
内容記述 | ニホンナシ‘埼玉8’[Pyrus pyrifolia(Burm. F.)],マメナシ‘愛知マメナシ’(P. calleryana Decne.),ニホンナシとイワテヤマナシとの交雑種‘岩手7号’(P. pyrifolia × P. ussuriensis var. aromatica)およびトヨトミナシ(P. mikawana Koidz.)の自然交雑実生を用いて,染色体の蛍光染色を実施した.供試全系統の染色体数は 2n = 34 であった.クロモマイシン A3(CMA)染色を行うと,全系統で4本の染色体の端部に CMA+ バンドが確認できた.4'-6-ディアミディノ-2-フェニルインドール二塩酸塩 (DAPI)− バンドの位置は CMA+ バンドと一致した.続いて,蛍光 in situ ハイブリダイゼーション(FISH)を‘おさゴールド’(Pyrus pyrifolia)およびトヨトミナシ自然交雑実生を用いて行った.6ケ所の 18S-5.8S-25S rDNA 遺伝子のうち,4ケ所は CMA+/DAPI− バンドの位置と一致した.5S rDNA 遺伝子は,2本の染色体の動原体近傍に確認できた.動原体近傍の2ケ所の 5S rDNA と端部の6ケ所の 18S-5.8S-25S rDNA 遺伝子は,各々別の染色体に存在した. | |||||
内容記述言語 | ja | |||||
収録雑誌名 |
en : Journal of the Japanese Society for Horticultural Science 巻 81, 号 1, p. 35-40, 発行日 2012 |
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作成日 | ||||||
日付 | 2012-01-01 | |||||
日付タイプ | Issued | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
ISSN | 18823351 | |||||
NII書誌ID(雑誌) | ||||||
収録物識別子タイプ | NCID | |||||
NC ID | AA12177046 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
DOI | https://doi.org/10.2503/jjshs1.81.35 | |||||
権利 | ||||||
権利情報の言語 | ja | |||||
権利情報 | 園芸学会 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
NDC | ||||||
主題Scheme | NDC | |||||
主題 | 625 | |||||
公開者・出版者 | ||||||
出版者言語 | ja | |||||
出版者 | 園芸学会 |