{"_buckets": {"deposit": "fdfc91f4-6b5c-4f99-8119-c7e6b400f128"}, "_deposit": {"created_by": 3, "id": "1029", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "1029"}, "status": "published"}, "_oai": {"id": "oai:ir.kagoshima-u.ac.jp:00001029", "sets": ["80"]}, "author_link": ["6519", "117817", "117818", "117815", "117814", "117816", "6517", "6518", "117813"], "item_8_alternative_title_18": {"attribute_name": "別言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Comprehensive regulatory system of PAMPs/DAMPs by TM-PC/EPCR system"}]}, "item_8_alternative_title_19": {"attribute_name": "タイトルよみ", "attribute_value_mlt": [{"subitem_alternative_title": "PAMPs-DAMPs ノ ホウカツテキ セイギョ システム トシテノ TM-PC/EPCR ソウチ"}]}, "item_8_date_6": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2013-06-21"}]}, "item_8_description_4": {"attribute_name": "要約(Abstract)", "attribute_value_mlt": [{"subitem_description": "2012-2012年度科学研究費助成事業(科学研究費補助金(挑戦的萌芽研究)研究成果報告書　課題番号：24659798　研究代表者：丸山征郎(鹿児島大学・大学院医歯学総合研究科・特任教授)", "subitem_description_type": "Other"}, {"subitem_description": "培養血管内皮細胞上のトロンボモジュリン（TM）分子のN末端レクチン様ドメインにはDAMPs：HMGB1, histones が, PAMPs：エンドトキシン（LPS）が結合して、それぞれそれらの活性を制御した。そこで今回の研究では特にHMGB1のダイナミズムについて研究した。TMのN末端のレクチン様ドメインに結合したHMGB1は、トロンビン・TM複合体によって、N末端のArg(10)-Gly(11)部位で切断されHMGB1は遊離されて、活性の低下したdes-HMGB1が生成された。このdes体はintact HMGB1の受容体（RAGE, TLRs-2,-4）への結合を阻害したことから、体内ではHMGB1とその受容体系のネガティブフィードバック系を形成しているものと推定された。そこでこのdes-HMGB1を即位的に認識するELISAを樹立した。このアッセイ法は９０％のdes-HMGB1を検出したが、１０％はintact HMGB1がコンタミネーションを起こしていた。本方法で諸疾患、特に遺伝子組換え体のＴＭで治療された患者の血中のdes-HMGB1した。遺伝子組換えTM投与で、速やかにdes-HMGB1が上昇する患者検体が観られた。しかし遺伝子組換えTMの投与でもdes-HMGB1の上昇しない症例、あるいはただちに低下する例などが観察され、遺伝子組換えTM治療に対し、レスポンダーと非レスポンダーがあること、あるいはdes-HMGB1の体内ターンオーバーの個体差の存在が推定された。", "subitem_description_type": "Other"}, {"subitem_description": "Endothelial thrombomodulin(TM) bounds DAMPs(Damage Associated Molecular Patterns);HMGB1,a representative DAMPs, histones. Moreover TM also bounds PAMPs(Pathogen Associated Molecular Patterns) ;lipopolysaccaride(LPS), a representative PAMPs, onto the lectin-like domain of the molecule and neutralizes their cytopathic activities, including proinflammatory and procoagulant activities. HMGB1 bound to TM, degraded by thrombin/TM complex and generated the N-terminus deleted HMGB1, named as des-HMGB1. Des-HMGB1 bound onto the receptors RAGE and TLR2-2, -4. However the binding affinity was weak compared to intact HMGB1, suggesting des-form of the molecule may configure a negative feedback loop among HMGB1 and its receptors regulating the diverse activities of HMGB1. Based on the findings, assay of des-HMGB1.may provide significant information in such morbid states including Disseminated Intravascular Coagulation(DIC), sepsis, systemic inflammatory response sybdrome(SIRS) and so on. Therefore we tried to establish specific assay method of des-HMGB1. We got specific monoclonal antibodies to react with the des-HMGB1. Using the monoclonal antibody, we established specific enzyme linked immune sorbent assay(ELISA). The ELISA successfully detected des-HMGB1 with 10% contamination of intact form. Des-HMGB1 was increased the serum from DIC, especially treated with recombinant TM. However the increased des-HMGB1 was decreased rapidly suggesting the rapid turn-over of the des-form. Moreover some patients showed no increased in des-HMGB1 even with recombinant TM treatment, suggesting of the presence or non-responder cases. Thus assessment of des-HMGB1 may be expected to provide a novel information in the DIC, SIRS, sepsis and so on with or without treatment.", "subitem_description_type": "Other"}]}, "item_8_full_name_2": {"attribute_name": "著者よみ", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "117813", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "マルヤマ, イクロウ"}]}, {"nameIdentifiers": [{"nameIdentifier": "117814", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "イトウ, タカシ"}]}, {"nameIdentifiers": [{"nameIdentifier": "117815", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "ハシグチ, テルト"}]}]}, "item_8_full_name_3": {"attribute_name": "別言語の著者", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "117816", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "MARUYAMA, Ikuro"}]}, {"nameIdentifiers": [{"nameIdentifier": "117817", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "ITO, Takashi"}]}, {"nameIdentifiers": [{"nameIdentifier": "117818", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "HASHIGUCHI, Teruto"}]}]}, "item_8_publisher_23": {"attribute_name": "公開者・出版者", "attribute_value_mlt": [{"subitem_publisher": "鹿児島大学"}]}, "item_8_subject_15": {"attribute_name": "NDC", "attribute_value_mlt": [{"subitem_subject": "494.27", "subitem_subject_scheme": "NDC"}]}, "item_8_text_24": {"attribute_name": "公開者よみ", "attribute_value_mlt": [{"subitem_text_value": "カゴシマ ダイガク"}]}, "item_8_text_25": {"attribute_name": "公開者別名", "attribute_value_mlt": [{"subitem_text_value": "Kagoshima University"}]}, "item_8_text_30": {"attribute_name": "NIIタイプ", "attribute_value_mlt": [{"subitem_text_value": "Research Paper"}]}, "item_8_text_41": {"attribute_name": "科研費番号 ", "attribute_value_mlt": [{"subitem_text_value": "24659798"}]}, "item_8_version_type_14": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "丸山, 征郎"}], "nameIdentifiers": [{"nameIdentifier": "6517", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000020082282", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}]}, {"creatorNames": [{"creatorName": "伊藤, 隆史"}], "nameIdentifiers": [{"nameIdentifier": "6518", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000020381171", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}]}, {"creatorNames": [{"creatorName": "橋口, 照人"}], "nameIdentifiers": [{"nameIdentifier": "6519", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000070250917", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-10-27"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "24659798.pdf", "filesize": [{"value": "180.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 180700.0, "url": {"label": "24659798.pdf", "url": "https://ir.kagoshima-u.ac.jp/record/1029/files/24659798.pdf"}, "version_id": "5eb9e38d-a8ec-4a56-845f-7b5329783c14"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "PAMPs", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DAMPs", "subitem_subject_scheme": "Other"}, {"subitem_subject": "トロンボモジュリン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "プロテインC", "subitem_subject_scheme": "Other"}, {"subitem_subject": "HMGB1", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "Pamps-DAMPsの包括的制御システムとしてのTM-PC/EPCR装置", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Pamps-DAMPsの包括的制御システムとしてのTM-PC/EPCR装置"}]}, "item_type_id": "8", "owner": "3", "path": ["80"], "permalink_uri": "http://hdl.handle.net/10232/19916", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-10-01"}, "publish_date": "2013-10-01", "publish_status": "0", "recid": "1029", "relation": {}, "relation_version_is_last": true, "title": ["Pamps-DAMPsの包括的制御システムとしてのTM-PC/EPCR装置"], "weko_shared_id": 3}