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海洋細菌のプロテアーゼに関する研究 III : プロテアーゼの精製と酵素学的性質について
http://hdl.handle.net/10232/13027
http://hdl.handle.net/10232/130278124db37-b096-4a29-8315-abe752cb5dd3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00040498_v26_p71-78.pdf (992.1 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-04-29 | |||||
| タイトル | ||||||
| タイトル | Studies on the Proteases of Marine Bacteria III : Purification and Some Enzymatic Properties of Proteases | |||||
| 言語 | en | |||||
| タイトル | ||||||
| タイトル | 海洋細菌のプロテアーゼに関する研究 III : プロテアーゼの精製と酵素学的性質について | |||||
| 言語 | ja | |||||
| 著者 |
坂田, 泰造
× 坂田, 泰造× 住吉, 和郎× 柿本, 大壱 |
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| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 要約(Abstract) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Four components with casein hydrolysing activity were found in extracellular protease produced by a marine Pseudomonas sp. I-6. Each protease component was fractionated by use of DEAE-Sephadex A-50, Sephadex G-100 and polyacrylamide gel electrophoresis. Protease activity was determined by the digestion of casein in the reaction mixture for fractions obtained on column chromatography and on a casein agar plate for polyacrylamide gels, respectively. The method of a casein agar plate was found to be convenient to detect the protease activity on the gel. Protease fractions were alkaline proteases of which optimal pHs were from pH 10.0 to pH 11.0. Proteases F-IIa and F-IIb were very low in activity without Ca^++ ion but reactivated with the addition of Ca^++ ion remarkably, whereas the activities of F-Ia and F-Ib were unaffected or slightly affected by Ca^++ ion. Proteases of this strain were stabilized to heat by Ca^++ ion but no substantial decrease in activity was observed during 6 hr incubation at 25 C regardless of the absence of Ca^++ ion. |
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| 言語 | en | |||||
| 収録雑誌名 |
ja : 鹿児島大学水産学部紀要 en : Memoirs of Faculty of Fisheries Kagoshima University 巻 26, p. 71-78, 発行日 1977-12-25 |
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| 作成日 | ||||||
| 日付 | 1977-12-25 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0453087X | |||||
| NII書誌ID（雑誌） | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00040498 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 663 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 言語 | en | |||||
