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  1. 掲載誌一覧
  2. 鹿児島大学水産学部紀要
  3. vol.26
  1. 水産学部
  2. 水産学部・紀要論文

Studies on the Proteases of Marine Bacteria III : Purification and Some Enzymatic Properties of Proteases

http://hdl.handle.net/10232/13027
http://hdl.handle.net/10232/13027
8124db37-b096-4a29-8315-abe752cb5dd3
名前 / ファイル ライセンス アクション
AN00040498_v26_p71-78.pdf AN00040498_v26_p71-78.pdf (992.1 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2012-04-29
タイトル
タイトル Studies on the Proteases of Marine Bacteria III : Purification and Some Enzymatic Properties of Proteases
別言語のタイトル
その他のタイトル 海洋細菌のプロテアーゼに関する研究 III : プロテアーゼの精製と酵素学的性質について
著者 SAKATA, Taizo

× SAKATA, Taizo

WEKO 104526

SAKATA, Taizo

Search repository
SUMIYOSHI, Kazuro

× SUMIYOSHI, Kazuro

WEKO 104527

SUMIYOSHI, Kazuro

Search repository
KAKIMOTO, Daiichi

× KAKIMOTO, Daiichi

WEKO 104528

KAKIMOTO, Daiichi

Search repository
別言語の著者
姓名 坂田, 泰造
別言語の著者
姓名 住吉, 和郎
別言語の著者
姓名 柿本, 大壱
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ departmental bulletin paper
要約(Abstract)
内容記述タイプ Other
内容記述 Four components with casein hydrolysing activity were found in extracellular protease produced by a marine Pseudomonas sp. I-6. Each protease component was fractionated by use
of DEAE-Sephadex A-50, Sephadex G-100 and polyacrylamide gel electrophoresis. Protease
activity was determined by the digestion of casein in the reaction mixture for fractions obtained
on column chromatography and on a casein agar plate for polyacrylamide gels, respectively.
The method of a casein agar plate was found to be convenient to detect the protease activity
on the gel.
Protease fractions were alkaline proteases of which optimal pHs were from pH 10.0 to pH
11.0. Proteases F-IIa and F-IIb were very low in activity without Ca^++ ion but reactivated
with the addition of Ca^++ ion remarkably, whereas the activities of F-Ia and F-Ib were unaffected or slightly affected by Ca^++ ion. Proteases of this strain were stabilized to heat by
Ca^++ ion but no substantial decrease in activity was observed during 6 hr incubation at 25 C
regardless of the absence of Ca^++ ion.
収録雑誌名 鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University

巻 26, p. 71-78
作成日
日付 1977-12-25
ISSN
収録物識別子タイプ ISSN
ISSN 0453087X
NII書誌ID(雑誌)
収録物識別子タイプ NCID
NC ID AN00040498
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
NDC
主題Scheme NDC
主題 663
公開者・出版者
出版者 鹿児島大学
公開者よみ
公開者よみ カゴシマ ダイガク
公開者別名
公開者別名 Kagoshima University
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