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雄鶏の血清蛋白質に関する免疫血清学的研究
http://hdl.handle.net/10232/18791
http://hdl.handle.net/10232/187915861d5d0-3ef5-4aff-b750-90e708a40bf3
| 名前 / ファイル | ライセンス | アクション |
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AN00040261_v13_p82-150.pdf (68.7 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2015-06-24 | |||||
| タイトル | ||||||
| タイトル | 雄鶏の血清蛋白質に関する免疫血清学的研究 | |||||
| タイトル言語 | ja | |||||
| タイトル | ||||||
| タイトル | Immunoserological Studies on the Cock Serum Protein | |||||
| タイトル言語 | en | |||||
| 著者 |
田代, 一男
× 田代, 一男 |
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| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Part 1 : Immunoserological Studies on that Change of serum protein which follows the growth of Cock |
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| 内容記述言語 | en | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | Studies on the relations between such phenomena as the growth and reproduction of cock and the change of serum protein were performed chiefly immunoserolo gically from the viewpoint of metabolism of endogenous hormone. The results obtained are summarized as follows : In the first chapter, the change of serum protein following the growth of cock was investigated immunoserologically. And the following facts were revealed; in MCS (1-day-old male chick serum) there is a specific protein fraction termed Fraction M that cannot be noticed in GCS (growing male chick serum) and ACS (adult cock serum), and the fraction continues to be recognized till it is about two weeks after hatching; in GCS and ACS there is another specific protein fraction termed Fraction A which cannot be noticed in MCS, and it appears during a period from about two to three weeks after hatching and afterwards it can be detected till it becomes an adult cock. Further the following facts were found as a result of agar gel precipitin ring test; there are immunoserologically at least three kinds of fraction in cock serum through the whole career from hatching till it becomes an adult cock; and there are in each stage specific protein fractions such as Fraction M and Fraction A, consequently from these facts the construction of serum proteins of MCS and ACS was supposed as shown in Fig. 11. No immunoserological specificity can be noticed between GCS and ACS. On one hand, each cock serum of various ages was electrophoretically investigated, but the existence of the specific protein fractions mentioned above could not be made clear. |
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| 内容記述言語 | en | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | In the second chapter, the serological nature of Fraction M and Fraction A was investigated. In order to examine the serological nature of the above mentioned specific protein fractions (M and A), anti-LHS (laying hen serum) and anti-CSS (cock semen serum) were prepared, and ACS, MCS, egg yolk Extracts, Albumin, LHS, CSS were used as antigenic substance. The results obtained are summarized as follows : Judging from the fact that the migration velocity of precipitin band in agar gel precipitin ring test shows linear regression, both in anti-MCS and anti-ACS, in conformity with migration formula, the chemical identification of each Fraction seemed possible. From the fact that Fraction M showed positive reaction to egg yolk extracts and LHS, it was presumed that Fraction M was very closely related to egg yolk extracts, and the fact made it clear that MCS, egg yolk and LHS have common protein fraction. On the other hand, it was found that Fraction A had nothing to do with albumin and MCS, showing positive reaction to CSS and LHS, and that reaction pattern was so similar that both ACS and CSS have the same construction of proteins. It was made clear from the above description that LHS holds Fraction M and Fraction A in common, then LHS was considered to be a union of both protein fractions of ACS and MCS, but the result of a later experiment caused the presumption to be modified and had it considered that LHS has a component (x~x') besides (MCS+ACS). It is considered that Fraction M or Fraction M+ (x~x') plays a pretty important part in the construction of LHS proteins, and that a comparatively small proportion is occupied by Fraction A. |
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| 内容記述言語 | en | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | It is confirmed that, from the result of reaction by anti-CSS, ACS and CSS shows a protein construction very similar to each other, and further that Fraction M has nothing to do with CSS. From these facts, it was presumed that Fraction M is closely related to egg yolk vitellin, and these facts showing that Fraction A is noticed in nothing but semen serum, ACS, LHS and so forth, led the author to presume that the Fraction A is related to a substance produced by Androgen. In the third chapter, therefore, the effects caused by sexual hormone injection, the removal of yolk sac, problems concerning the immunoserological specificity of serum of male chick dieted with much yolk and the physiological roles played by Fraction M in the earlier stage of chick were investigated. The results are summarized as follows : When Estrogen is injected into chicks and adult cocks, substance corresponding to Fraction M appears; when Androgen is injected into male day old chicks, substance corresponding to Fraction A appears; when yolk sac is removed, Fraction M makes regress and inversely, a surprisingly great quantity of Fraction M was noticed in the serum of chick dieted with much yolk. This experiment raised several problems concerning the effect of hormone. That is to say, when Estrogen is injected into the cock, Fraction M is supposed to have synergism and Fraction A has antagonism to Estrogen. When a much dose of Androgen is injected into the male day old chick, it is observed that its comb increases remarkably in weight a week after the injection compared with that at a normal stage, and that the testes, on the other hand, becomes atrophied. In this case, no substance corresponding to Fraction M is noticed in the serum, while some substance corresponding to Fraction A is noticed. This fact is a reverse phenomena to a normal chick serum in a case where no treatment is given, which is a very important fact. The point to be debated here is a serological difference between LHS and MCS. |
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| 内容記述言語 | en | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | It was previously mentioned that the construction of protein of laying hen serum was supposed to be ACS+MCS+(x~x'). Thereupon it is a very interesting point to know in what position and form and proportion the Fraction M exists in the protein fraction of laying hen. The following are the summarized results obtained from serum reaction : Antigenic substances showing positive reaction to Fraction M are MCS, LHS, egg yolk extracts, estrogen-treated Cock serum, serum of male chick dieted with much yolk and so forth, while antigenic substances showing positive reaction to anti-LHS are MCS, ACS and LHS. When the anti-LHS is absorbed by MCS, LHS proved positive fraction; when the anti-LHS is absorbed by ACS, both MCS and LHS proved positive fraction. From these reactions, it is considered that a comparatively small proportion is occupied by protein fraction proper to ACS in LHS. When the anti-LHS is absorbed by MCS, Will it not be considered that ACS fraction is removed accompanyingly? This point is left unsolved. If the main constituent of serum protein proper to laying hen is protein complex of Lipoprotein, it is considered that it may be contained in (x~x'). It is a question to find where is a substance appearing correspondingly to Androgen injection, and probably it seems proper to think that it is contained in Fraction A itself. In any case, the (x~x') component is considered to be the main component of a specific protein of LHS, and it will be necessary that the relation between this component and MCS, and between Fraction A and Androgen will be fundamentally investigated. From the finding that anti-MCS injection impedes the growth of chick of 1~2 weeks old in a certain degree, it is confirmed that Fraction M plays a physiological role in an earlier growth of chick. But it has not been elucidated by the author in detail whether it is so important protein fraction as to control the growth of chick. |
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| 内容記述言語 | en | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | Part 2 : Immunoserological Studies on the Hypophysial Gonadotropin of Cock | |||||
| 内容記述言語 | en | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The result obtained by investigating the change of serum protein that accompanies the growth of cock from the viewpoint of metabolism of endogenous hormone has already been mentioned in Part I. There it was reported that the phase of growth was divided into three stages and using the serum at each stage as antigen, the specific serological difference between the stages was elucidated and that the protein fraction proper to each stage was deeply related to Estrogen or Androgen. The existence of pituitary growth hormone as a great element having a share in the growth of an animal cannot be ignored. As the existence of growth hormone in the cock was not made clear, the level of GTH (Gonado-trophic hormone) in blood was going to be immunoserologically investigated fundamentally. Now, as whether the gonadotrophic substance of cock pituitary gland had antigenicity or not had not been fundamentally elucidated, the author has mad up his mind to examine that point. As it is said that there is much to be debated whether there is anti-hormone formation for protein hormone, the problem was considered bibliographically in the first chapter and it was pointed out that the formation of antihormone was inclined to be affirmed. In the second chapter, it was reported that anti-hormone formation for cock hypophysial gonadotrophic hormone had been affirmed by means of bio-assay. The cock anterior pituitary acetone dried powder which had been suspended in oil adjuvant was used as antigen. When a cock was injected with 1~2cc. of antiserum at the same time with anterior-pituitary acetone powdered preparation, it was noticed that 40~50% of gonadotrophic activity was inhibited in any case and that antiserum was higher in its inhibitory action in a case of 2cc. injection than 1cc. |
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| 内容記述言語 | en | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | When a cock was used as an animal for immunization or when antigenserum was not suspended in oil Adjuvant, such antihormone formation could not be proved. The author attempted an immunoserological assay of hormone contained in blood with antihormone as an index. From the necessity of measuring antibody titer in the process of immunization, the anterior pituitary powder which was dissolved in MCS was used as antigenic liquid, and immunoassay was attempted by means of the technique of precipitin ring test and agar gel precipitin test, by using immune rabbit serum obtained. The result showed, as reported in the third chapter, that a certain degree of specific reaction could be noticed, and the immunoassay of cock gonadotrophic hormone was considered to be difficult for the time being. But immunoassay for GTH is thought possible if any specific substance could be removed from anti-CHG rabbit serum and if such conditions could be fulfilled as the constancy of anti GTH activity in antiserum and the raising the purity of the fractionation of gonadotrophic substance. On the other hand, it was generally accepted that frequent injection of protein hormone would produce an antibody, namely, antihormone, which fact was confirmed by the author in his experiment. The fact has been one of the greatest obstacles in injecting protein hormone for test or for the object of curing. Therefore, when several kinds of investigation were made as to the method of neutralizing the antihormone, it was made clear by treating with serum in an adjuvant manner that the anti-serum containing antihormone has proper antigenicity. The results obtained by experiment in twice and some discussions concerning them were reported in the fourth chapter. What is to say, when the inhibition of antihormone and degree of neutralization were expressed by the percentage of neutralization, the titer was found to be 50~60% or so. If some method of increasing the amount of anti-antiserum injected and heightening the antibody titer is to be employed, it seems that a more effective inhibition of antihormone and neutralizing action will appear, and the method will have a very important meaning in the way of clinical application and thereby it is thought possible to remove one of the obstacles to application of protein hormone. |
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| 内容記述言語 | en | |||||
| 収録雑誌名 |
ja : 鹿児島大学教育学部研究紀要. 自然科学編 en : Bulletin of the Faculty of Education, Kagoshima University. Natural science 巻 13, p. 82-150, 発行日 1961-01-01 |
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| 作成日 | ||||||
| 日付 | 1961-01-01 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| ISSN | 03896692 | |||||
| NII書誌ID（雑誌） | ||||||
| 収録物識別子タイプ | NCID | |||||
| NC ID | AN00040261 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 481 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 出版者言語 | en | |||||
