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Pharmacological inhibition of the Hedgehog pathway prevents human rhabdomyosarcoma cell growth
http://hdl.handle.net/10232/19983
http://hdl.handle.net/10232/19983657605a3-25e0-4759-9c1f-28739d3c59df
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Pharmacological inhibition of the Hedgehog pathway prevents human rhabdomyosarcoma cell growth.pdf (666.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2014-02-10 | |||||
| タイトル | ||||||
| タイトル | Pharmacological inhibition of the Hedgehog pathway prevents human rhabdomyosarcoma cell growth | |||||
| タイトル言語 | en | |||||
| 著者 |
川畑, 直也
× 川畑, 直也× 井尻, 幸成× 石堂, 康弘× 山元, 拓哉× 永尾, 宗子× 永野, 聡× 前田, 真吾× 小宮, 節郎× 瀬戸口, 啓夫 |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | rhabdomyosarcoma | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Hedgehog | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | cyclopamine | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | GANT61 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The Hedgehog pathway functions as an organizer in embryonic development. Recent studies have shown that mutation of the PTCH1 gene involved in the Hedgehog path-way affects rhabdomyosarcoma development. However, the expression of Hedgehog pathway molecules in human rhabdomyosarcoma cells has not been well clarified. In addition, the effect of pharmacological inhibition of the Hedgehog pathway is not known. We investigated the expression of the genes involved in the Hedgehog pathway using human rhabdomyosarcoma cell lines and biopsy specimens. Further, we evaluated the effect of pharmacological inhibition of the Hedgehog pathway using cyclopamine or GANT61 by WST assay, cell proliferation assay and cell death detection assay. Real-time PCR revealed that human rhabdomyosarcoma cell lines and biopsy specimens overexpressed the following genes: Sonic hedgehog, Indian hedgehog, Desert hedgehog, PTCH1, SMO, GLI1, GLI2 and ULK3. Immunohistochemistry revealed that rhabdomyosarcoma cell lines and biopsy specimens expressed SMO and GLI2. Inhibition of SMO by cyclopamine slowed the growth of human rhabdomyosarcoma cell lines. Similarly, inhibition of GLI by GANT61 slowed the growth of human rhabdomyosarcoma cell lines. Inhibition of cell proliferation and apoptotic cell death together prevented the growth of rhabdomyosarcoma cells by cyclopamine and GANT61 treatment. Our findings suggest that pharmacological inhibition of the Hedgehog pathway may be a useful approach for treating rhabdomyosarcoma patients. | |||||
| 内容記述言語 | en | |||||
| 収録雑誌名 |
en : International Journal of Oncology 巻 39, 号 4, p. 899-906, 発行日 2011 |
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| 作成日 | ||||||
| 日付 | 2011-06-14 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| ISSN | 10196439 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| DOI | https://doi.org/10.3892/ijo.2011.1076 | |||||
| 権利 | ||||||
| 権利情報の言語 | en | |||||
| 権利情報 | Spandidos Publications | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 494.5 | |||||
| 公開者・出版者 | ||||||
| 出版者 | Spandidos Publications | |||||
| 出版者言語 | en | |||||
| 関連（relation）（relationType設定なし） | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.spandidos-publications.com/ijo/39/4/899 | |||||
