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アイテム
Orexin neurons are indispensable for prostaglandin E2-induced fever and defence against environmental cooling in mice
http://hdl.handle.net/10232/25482
http://hdl.handle.net/10232/25482d06220b1-1763-4160-a259-5a9ade12e71a
| 名前 / ファイル | ライセンス | アクション |
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Diss_高橋_佳子_ISK297_2014.pdf (28.3 MB)
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Abstract_高橋_佳子_4505810238_2014.pdf (90.3 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2015-10-29 | |||||
| タイトル | ||||||
| タイトル | Orexin neurons are indispensable for prostaglandin E2-induced fever and defence against environmental cooling in mice | |||||
| タイトル言語 | en | |||||
| 著者 |
高橋, 佳子
× 高橋, 佳子 |
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| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | We recently showed using prepro-orexin knockout (ORX-KO) mice and orexin neuron-ablated (ORX-AB) mice that orexin neurons in the hypothalamus, but not orexin peptides per se, are indispensable for stress-induced thermogenesis. In order to examine whether orexin neurons are more generally involved in central thermoregulatory mechanisms or not, we applied other forms of thermogenic perturbations, including brain PGE2 injections which mimic inflammatory fever and environmental cold exposure, to ORX-KO mice, ORX-AB mice, and their wild-type (WT) litter mates. We found that ORX-AB mice, but not ORX-KO mice, exhibited a blunted PGE2-induced fever and intolerance to cold (5°C) exposure, and these findings were similar to the results previously obtained with stress-induced thermogenesis. PGE2-induced shivering was also attenuated in ORX-AB mice. Both mutants responded similarly to environmental heating (39°C). In WT and ORX-KO mice, the administration of PGE2 and cold exposure activated orexin neurons, as revealed by increased levels of expression of c-fos. Injection of retrograde tracer into the medullary raphe nucleus revealed direct and indirect projection from the orexin neurons, of which the latter seemed to be preserved in the ORX-AB mice. In addition, we found that glutamate receptor antagonists (D-(-)-2-amino-5-phosphonopentanoic acid and 6-cyano-7-nitroquinoxaline-2,3-dione) but not orexin receptor antagonists (SB334867 and OX2 29) successfully inhibited PGE2-induced fever in WT mice. These results suggest that orexin neurons are important in general thermogenic processes, and their importance is not restricted to stress-induced thermogenesis. In addition, these results indicate the possible involvement of glutamate in orexin neurons implicated in PGE2-induced fever. Yoshiko Takahashi, Wei Zhang, Kohei Sameshima, Chiharu Kuroki, Ami Matsumoto, Jinko Sunanaga, Yu Kono, Takeshi Sakurai, Yuichi Kanmura, and Tomoyuki Kuwaki Orexin neurons are indispensable for prostaglandin E2-induced fever and defence against environmental cooling in mice Journal of Physiology, 2013, 591(Pt 22), p.5623–5643 doi: 10.1113/jphysiol.2013.261271 |
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| 内容記述言語 | en | |||||
| 作成日 | ||||||
| 日付 | 2015-09-14 | |||||
| 日付タイプ | Collected | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 490 | |||||
| ファイル（説明） | ||||||
| 内容記述 | 博士論文全文, 博士論文要旨 | |||||
| 公開者・出版者 | ||||||
| 出版者 | Physiological Society | |||||
| 出版者言語 | en | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | カゴシマ ダイガク | |||||
| 出版者言語 | ja-Kana | |||||
| 備考 | ||||||
| 備考 | 【指導教員:上村裕一】 | |||||
| date.appl | ||||||
| appl | 【学位申請日】2014-04-02 | |||||
| 学位名 | ||||||
| 学位名の言語 | ja | |||||
| 学位名 | 博士(医学) | |||||
| 学位名 | ||||||
| 学位名の言語 | en | |||||
| 学位名 | Doctor of Philosophy in Medical Science | |||||
| 学位授与機関名 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 17701 | |||||
| 学位授与機関名の言語 | ja | |||||
| 学位授与機関名 | 鹿児島大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2014-07-11 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲総研第297号 | |||||
