{"_buckets": {"deposit": "842aa467-c328-465e-9e95-191de20a82f4"}, "_deposit": {"created_by": 24, "id": "14413", "owners": [24], "pid": {"revision_id": 0, "type": "depid", "value": "14413"}, "status": "published"}, "_oai": {"id": "oai:ir.kagoshima-u.ac.jp:00014413", "sets": ["86"]}, "author_link": ["132048", "132047", "132049"], "item_5_alternative_title_18": {"attribute_name": "別言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "尿路由来大腸菌のフルオロキノロン耐性遺伝子についての解析"}]}, "item_5_biblio_info_5": {"attribute_name": "収録雑誌名", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "368", "bibliographicPageStart": "360", "bibliographicVolumeNumber": "19", "bibliographic_titles": [{"bibliographic_title": "INTERNATIONAL JOURNAL OF UROLOGY"}]}]}, "item_5_date_granted_54": {"attribute_name": "学位授与年月日 ", "attribute_value_mlt": [{"subitem_dategranted": "2014-11-17"}]}, "item_5_degree_grantor_53": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "鹿児島大学"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "17701", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_5_degree_name_42": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（医学）Doctor of Philosophy in Medical Science"}]}, "item_5_description_17": {"attribute_name": "ファイル（説明）", "attribute_value_mlt": [{"subitem_description": "博士論文全文", "subitem_description_type": "Other"}, {"subitem_description": "博士論文要旨", "subitem_description_type": "Other"}, {"subitem_description": "最終試験結果の要旨", "subitem_description_type": "Other"}, {"subitem_description": "論文審査の要旨", "subitem_description_type": "Other"}]}, "item_5_description_4": {"attribute_name": "要約(Abstract)", "attribute_value_mlt": [{"subitem_description": "Objectives: Coincident with their worldwide use, resistance to fluoroquinolones in Escherichia coli has increased.To identify the gene expression profiles underlying fluoroquinolone resistance, we carried out genome-wide transcriptome analysis of fluoroquinolone-sensitiveE. coli.\nMethods: Four fluoroquinolone-sensitive E. coli and five fluoroquinolone-resistant E. coli clinical isolates were subjected to complementary deoxyribonucleic acid icroarray analysis. Some upregulated genes’ expression was verified by real-time polymerase chain reaction using 104 E. coli clinical isolates, and minimum inhibitory concentration tests were carried out by using their transformants.\nResults: A total of 40 genes were significantly upregulated influoroquinolone-resistant E. coli isolates (P\u003c0.05). The expression of phage shock protein operons, which are involved in biofilm formation, was markedly upregulated in our profile of fluoroquinolone-resistant E. coli. One of the phage shock protein operons, pspC, was significantly upregulated in 50 fluoroquinolone-resistant E. coli isolates (P\u003c0.0001). \nThe expression of type I fimbriae genes, which are pilus operons involved in biofilm formation, were markedly downregulated in fluoroquinolone-resistant E. coli. Deoxyri-bonucleic acid adenine methyltransferase (dam), which represses type I fimbriae genes, was significantly upregulated in the clinical fluoroquinolone-resistant E. coli iso-lates (P=0.007). We established pspC- and dam-expressing E. coli transformants from fluoroquinolone-sensitive E. coli, and the minimum inhibitory concentration tests showed that the transformants acquired fluoroquinolone resistance, suggesting that upregulation of these genes contributes to acquiring fluoroquinolone resistance.\nConclusions: Upregulation of psp operones and dam underlying pilus operons down-regulation might be associated with fluoroquinolone resistance in E. coli.", "subitem_description_type": "Other"}]}, "item_5_dissertation_number_55": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲総研第308号"}]}, "item_5_full_name_2": {"attribute_name": "著者よみ", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "132048", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "ヤマネ, タカシ"}]}]}, "item_5_full_name_3": {"attribute_name": "別言語の著者", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "132049", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "YAMANE, Takashi"}]}]}, "item_5_publisher_23": {"attribute_name": "公開者・出版者", "attribute_value_mlt": [{"subitem_publisher": "The Japanese Urological Association"}, {"subitem_publisher": "鹿児島大学"}]}, "item_5_relation_13": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "INTERNATIONAL JOURNAL OF UROLOGY"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://onlinelibrary.wiley.com/journal/14422042", "subitem_relation_type_select": "URI"}}]}, "item_5_relation_36": {"attribute_name": "関連（hasPart）", "attribute_value_mlt": [{"subitem_relation_type": "hasPart", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1111/j.1442-2042.2011.02933.x", "subitem_relation_type_select": "URI"}}]}, "item_5_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2011 The Japanese Urological Association"}]}, "item_5_subject_15": {"attribute_name": "NDC", "attribute_value_mlt": [{"subitem_subject": "491", "subitem_subject_scheme": "NDC"}]}, "item_5_text_24": {"attribute_name": "公開者よみ", "attribute_value_mlt": [{"subitem_text_value": "カゴシマ ダイガク"}]}, "item_5_text_25": {"attribute_name": "公開者別名", "attribute_value_mlt": [{"subitem_text_value": "Kagoshima University"}]}, "item_5_text_30": {"attribute_name": "NIIタイプ", "attribute_value_mlt": [{"subitem_text_value": "Thesis or Dissertation"}]}, "item_5_text_44": {"attribute_name": "備考", "attribute_value_mlt": [{"subitem_text_value": "【指導教員:中川昌之】"}]}, "item_5_version_type_14": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", 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resistance", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "microarray", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Genome-wide transcriptome analysis of fluoroquinolone resistance in clinical isolates of Escherichia coli", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Genome-wide transcriptome analysis of fluoroquinolone resistance in clinical isolates of Escherichia coli"}]}, "item_type_id": "5", "owner": "24", "path": ["86"], "permalink_uri": "http://hdl.handle.net/10232/00030317", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-08-01"}, "publish_date": "2018-08-01", 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