{"_buckets": {"deposit": "46c7ae6b-1a2e-4871-a2df-0960f510e2ee"}, "_deposit": {"created_by": 24, "id": "15603", "owners": [24], "pid": {"revision_id": 0, "type": "depid", "value": "15603"}, "status": "published"}, "_oai": {"id": "oai:ir.kagoshima-u.ac.jp:00015603", "sets": ["86"]}, "author_link": ["138133", "138134", "138135"], "item_5_alternative_title_18": {"attribute_name": "別言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "5-Aza-2-deoxycytidineはthymidine phosphorylaseの脱メチル化を介して5-fluorouracilの"}]}, "item_5_date_6": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2020-10-08"}]}, "item_5_date_granted_54": {"attribute_name": "学位授与年月日 ", "attribute_value_mlt": [{"subitem_dategranted": "2020-10-16"}]}, "item_5_degree_grantor_53": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "鹿児島大学"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "17701", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_5_degree_name_42": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（医学）Doctor of Philosophy in Medical Science"}]}, "item_5_description_17": {"attribute_name": "ファイル（説明）", "attribute_value_mlt": [{"subitem_description": "博士論文全文", "subitem_description_type": "Other"}, {"subitem_description": "博士論文要旨", "subitem_description_type": "Other"}, {"subitem_description": "最終試験結果の要旨", "subitem_description_type": "Other"}, {"subitem_description": "論文審査の要旨", "subitem_description_type": "Other"}]}, "item_5_description_4": {"attribute_name": "要約(Abstract)", "attribute_value_mlt": [{"subitem_description": "Background/Aim: 5-Aza-2-deoxycytidine (5-Aza-CdR) enhances the sensitivity to 5-fluorouracil (5-FU), but the molecular mechanism is not fully understood. The aim of this study was to investigate the molecular mechanism that enhances the sensitivity to 5-FU treated with 5-Aza-CdR via thymidine phosphorylase (TP). Materials and Methods: The sensitivity to drugs was determined on several cancer cell lines by the MTT assay. Protein and mRNA levels were examined by immunoblot and RT-PCR, respectively. Gene silencing, binding of Sp1 to DNA and methylation of DNA was performed by siRNA, ChIP assay and sodium bisulfate genomic sequencing, respectively. Results: Sp1-binding sites in the TP promoter were methylated in epidermoid carcinoma. 5-Aza-CdR demethylated Sp1-binding sites and enhanced sensitivity to 5-FU. Conclusion: Demethylation of Sp1-binding sites by 5-Aza-CdR was a key factor enhancing 5-FU sensitivity, which may enable more effective treatments for cancer patients with the combination of 5-Aza-CdR and 5-FU.", "subitem_description_type": "Other"}, {"subitem_description": "YUKIHIKO NISHIZAWA, RYUJI IKEDA, MASATATSU YAMAMOTO, KOHICHI KAWAHARA, YOSHINARI SHINSATO, KENTARO MINAMI, MINA NITTA, HIDEYUKI TERAZONO, SHIN-ICHI AKIYAMA, TATSUHIKO FURUKAWA and YASUO TAKEDA\n5-Aza-2-deoxycytidine Enhances the Sensitivity of 5-Fluorouracil by Demethylation of the Thymidine Phosphorylase Promoter\nAnticancer Research, 2019, 39(8) p.4129-4136\nDOI: https://doi.org/10.21873/anticanres.13571", "subitem_description_type": "Other"}]}, "item_5_dissertation_number_55": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "乙総論第40号"}]}, "item_5_full_name_2": {"attribute_name": "著者よみ", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "138134", 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