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Studies on Muscle Proteins III. Behaviours of Actins in 7M Urea-Containing Buffer Solution
http://hdl.handle.net/10232/9481
http://hdl.handle.net/10232/9481ce01e591-fa7e-42e2-978d-b60c9ae6430e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AA00732376_v7n1_p47-53.pdf (1.6 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-03-03 | |||||
| タイトル | ||||||
| タイトル | Studies on Muscle Proteins III. Behaviours of Actins in 7M Urea-Containing Buffer Solution | |||||
| 著者 |
KAKO, Yoshitaka
× KAKO, Yoshitaka |
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| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The preparations of G- and F-actin from beef, pork and chicken were performed by the methods of Straub and Krans et al. and they were analyzed through DEAE cellulose column chromatography and starch gel electrophoresis in 7M urea containing buffer solution (pH 8.6) by the method almost the same as those described in the previous paper. From the results obtained out of chromatography, it was evident that the principal peak of F-actin (not G-actin) corresponded to that of the maximal peak of the chromatogram of the meat-urea solution in all kinds of the animal tested, as was noted in the previous study. And from the results of starch gel electrophoresis, the conspicuous two bands, situated in the middle of each run, which had been found in the patterns of raw and cooked meat-urea solutions, were found in F-actin, at the position identical with the one mentioned above. Besides, the fraction corresponded to the principal peak on the DEAE cellulose column chromatogram was collected and condensed, and the component in it was analyzed by SGE technique. The result showed the perfect coincidence of the both components, that is, one was the component that appeared on the chromatogram as a principal peak, the other was the component which was revealed on the SGE pattern as two conspicuous bands. Finally, to give one more certainty to this conclusion above, the presence of the bound ADP in the component which is specific to F-actin, was investigated by measuring the absorbance at 260 mμ through DEAE cellulose column chromatography. The result proved the obvious presence of the bound ADP. |
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| 収録雑誌名 |
Memoirs of the Faculty of Agriculture, Kagoshima University 巻 7, 号 1, p. 47-53 |
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| 作成日 | ||||||
| 日付 | 1969-03-25 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| ISSN | 04530853 | |||||
| NII書誌ID（雑誌） | ||||||
| 収録物識別子タイプ | NCID | |||||
| NC ID | AA00732376 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 464 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 公開者よみ | ||||||
| 公開者よみ | カゴシマ ダイガク | |||||
| 公開者別名 | ||||||
| 公開者別名 | Kagoshima University | |||||
| 関連（relation）（relationType設定なし） | ||||||
| 関連タイトル（relation） | http://hdl.handle.net/10232/9478 | |||||
| 備考 | ||||||
| 備考 | Has errata: http://hdl.handle.net/10232/9478 | |||||
