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LPS profiles of TF7 and its mutants were revealed by using SDS-PAGE\nand immunoblotting techniques. One type of the mutants was found to lose O-saccharide chain\nof LPS completely and concomitantly to change to be serum sensitive. The other type of the\nmutants displayed to produce less amount of O-side chains than TF7 and to be resistant to\nrabbit serum conditionally. When the cells were grown on TSA containing SDS (TSA-SDS)\nor MacConkey agars, the former mutants exhibited higher sensitivity, while the latter turned to\nbe serum sensitive considerably. The parent strain TF7 were not significantly different in\nrespect with serum susceptibility even if grown on TSA-SDS agar. 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Serum Sensitivity of a Fish Pathogenic Strain and Its LPS Mutants of Aeromonas hydrophila
http://hdl.handle.net/10232/13379
http://hdl.handle.net/10232/133790aead340-4c1d-4ef7-b64a-f1080c839efb
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2012-05-07 | |||||
タイトル | ||||||
タイトル | Serum Sensitivity of a Fish Pathogenic Strain and Its LPS Mutants of Aeromonas hydrophila | |||||
別言語のタイトル | ||||||
その他のタイトル | エロモナス・ハイドロフィラの病原株とLPS変異菌株の血清感受性 | |||||
著者 |
SAKATA, Taizo
× SAKATA, Taizo |
|||||
別言語の著者 | ||||||
姓名 | 坂田, 泰造 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Aeromonas hydrophila | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | serum sensitivity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | LPS mutants | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | LPS profiles | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | immunoblot | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
要約(Abstract) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Two types of LPS mutants were isolated from a virulent strain, TF7 of Aeromonas hydrophila after UV irradiation. LPS profiles of TF7 and its mutants were revealed by using SDS-PAGE and immunoblotting techniques. One type of the mutants was found to lose O-saccharide chain of LPS completely and concomitantly to change to be serum sensitive. The other type of the mutants displayed to produce less amount of O-side chains than TF7 and to be resistant to rabbit serum conditionally. When the cells were grown on TSA containing SDS (TSA-SDS) or MacConkey agars, the former mutants exhibited higher sensitivity, while the latter turned to be serum sensitive considerably. The parent strain TF7 were not significantly different in respect with serum susceptibility even if grown on TSA-SDS agar. The most distinguishable feature of whole cell protein profiles on SDS-PAGE was observed as the decrease of 49 Kd band concomitantly with increase 41 Kd band for both the parent and mutant strains grown on TSA-SDS agar. |
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収録雑誌名 |
鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University 巻 37, p. 147-159 |
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作成日 | ||||||
日付 | 1988-12-25 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
ISSN | 0453087X | |||||
NII書誌ID(雑誌) | ||||||
収録物識別子タイプ | NCID | |||||
NC ID | AN00040498 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
NDC | ||||||
主題Scheme | NDC | |||||
主題 | 663 | |||||
公開者・出版者 | ||||||
出版者 | 鹿児島大学 | |||||
公開者よみ | ||||||
公開者よみ | カゴシマ ダイガク | |||||
公開者別名 | ||||||
公開者別名 | Kagoshima University |