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The knock-down of overexpressed EZH2 and BMI-1 does not prevent osteosarcoma growth
http://hdl.handle.net/10232/19978
http://hdl.handle.net/10232/19978d9ecd335-d191-4c9c-be82-5b948e926728
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
The knock-down of overexpressed EZH2 and BMI-1 does not prevent osteosarcoma growth.pdf (367.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2014-02-10 | |||||
| タイトル | ||||||
| タイトル | The knock-down of overexpressed EZH2 and BMI-1 does not prevent osteosarcoma growth | |||||
| タイトル言語 | en | |||||
| 著者 |
佐々木, 裕美
× 佐々木, 裕美× 瀬戸口, 啓夫× 松野下, 幸弘× Gao, Hui× 廣津, 匡隆× 小宮, 節郎 |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | polycomb protein | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | osteosarcoma | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | EZH2 | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | BMI-1 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Polycomb group proteins control the transcriptional memory of cells by maintaining the stable silencing of specific sets of genes through chromatin modifications. Polycomb group protein complexes control gene repression through recruitment of histone deacetylase. This recruitment leads to trimethylation of Lys27 of histone H3 (H3K27). Histone H3K27 trimethylation is a property of stably silenced heterochromatin. EZH2 and BMI-1 are pivotal components of polycomb group protein complexes. Increased EZH2 levels have been found in several malignancies and reported as a molecular biomarker of poor prognosis. Similarly, BMI-1 has also been found to be associated with malignant transformation. In addition, inhibition of EZH2 or BMI-1 inhibits the growth of various types of malignancies. The expression of BMI-1 and EZH2 in human osteosarcoma has not been clearly determined. We examined the potential involvement of aberrant polycomb group protein expression in the pathogenesis of osteosarcoma. Real-time PCR revealed that expression of EZH2 in 143B, HOS, NOS-1 and Saos2 was increased compared to normal osteoblasts. BMI-1 was also up-regulated in 143B, HOS and NOS-1. Expression of EZH2 and BMI-1 were up-regulated in osteosarcoma patient biopsy specimens compared to normal bone. Immunohistochemical examinations showed that EZH2 and BMI-1 were up-regulated in osteosarcoma cells and that trimethylation of histone H3K27 was increased. We examined the effects of knock down of EZH2 and BMI-1 by shRNA. Unexpectedly, the knock-down of EZH2 and BMI-1 did not prevent osteosarcoma growth either in vitro or in vivo. Our findings suggest that EZH2 and BMI-1 may be tumorassociated antigens of osteosarcoma, but are not useful molecular targets of osteosarcoma treatment. | |||||
| 内容記述言語 | en | |||||
| 収録雑誌名 |
en : Oncology Reports 巻 23, 号 3, p. 677-684, 発行日 2010 |
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| 作成日 | ||||||
| 日付 | 2010-03-01 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| ISSN | 1021335X | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| DOI | https://doi.org/10.3892/or_00000684 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 493.6 | |||||
| 公開者・出版者 | ||||||
| 出版者 | Spandidos Publications | |||||
| 出版者言語 | en | |||||
| 関連（relation）（relationType設定なし） | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.spandidos-publications.com/or/23/3/677 | |||||
