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  1. 連合農学研究科
  2. 連合農学研究科・博士論文

海産ラビリンチュラ類の溶菌活性に関する研究

http://hdl.handle.net/10232/4661
http://hdl.handle.net/10232/4661
1378df90-3f70-45cc-9126-4fd60fad9a69
名前 / ファイル ライセンス アクション
renken611.pdf renken611.pdf (287.1 kB)
Wahid-D.pdf Wahid-D.pdf (4.5 MB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2015-02-19
タイトル
タイトル Studies on Bacteriolytic Activities of Marine Labyrinthulids
タイトル言語 en
タイトル
タイトル 海産ラビリンチュラ類の溶菌活性に関する研究
タイトル言語 ja
著者 Md. イフテカルル ワヒド

× Md. イフテカルル ワヒド

WEKO 126251

en Md. Iftekharul Wahid

ja Md. イフテカルル ワヒド

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言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_46ec
資源タイプ thesis
要約
内容記述タイプ Other
内容記述 Thirteen strains of labyrinthulids were isolated from the coastal area of Kagoshima Bay, Kagoshima Prefecture, Japan and from Batan Bay, Panay Island, the Philippines by using diatom double-layer agar plates. The cells of labyrinthulid isolates grew axenically on the bacterial dead cells and extract plus egg yolk agar medium (NSBEY agar). Three representative isolates were demonstrated to belong to the labyrinthulid phylogenetic group (LPG) based on 18S rDNA sequence analysis. In the hydrolysis test of labyrinthulid isolates, it was found that they could hydrolyse macromolecular compounds like starch. Ten enzymes were detected by using API-ZYM Kit in four representative isolates. Three enzymes including alkaline phosphatase, leucine arylamidase and valine arylamidase were found in higher level in API-ZYM tests. As examined their bacteriolytic activity, it was found that they could lyse only the dead cells of Gram-negative bacteria and not those of Gram-positive bacteria during incubation in both agar and liquid media. The optimum temperature range for their bacteriolysis was from 25 to 31°C. Respiratory inhibitors such as sodium cyanide, dinitrophenol and sodium azide had repressed bacteriolysis activity but no impact on viability was found in Labyrinthula cells of a strain 00–Bat-05, Philippine isolate. Orange carotenoid pigment(s) was accumulated during stationary growth phase of strain 00-Bat-05, cultured in an L-shape tube containing a bacterial dead cell suspension and concomitantly rapid cell movement of developing zoospores was observed.
Leucine aminopeptidases (LAPs) from marine labyrinthulid strain 00-Bat-05 and thraustochytrid strain HR-3 cells were partially purified and characterized by enzymological properties. The optimum temperature of LAPs from both strains was 37°C. The thermostability of 00-Bat-05 LAP was indicated by having 80% of maximum activity after heat treatment at 60oC for 10 min, while LAP activity of strain HR-3 was completely inactivated at 60°C. LAP acvtivities from both strains were high at near pH 8.0. Both LAPs were inhibited by 1,10-phenanthroline, p-chloromercuribenzoic acid (PCMB), bestatin and sodium dodecyl sulphate (SDS), suggesting that both are SH-aminopeptidase. Enzyme activity of LAP from strain 00-Bat-05 was stimulated by Co2+ and inhibited by Zn2+, while that from HR-3 was inhibited by Co2+ and Zn2+. LAP of 00-Bat-05 had a high specificity for L-leucine-p-nitroanilide but HR-3 enzyme showed relatively broad specificity for p-nitroanilide derivatives of L-amino acids. Partially purified LAP enzyme, from labyrinthulid sp. strain 00-Bat-05, showed the bacteriolysis activity against the dead cells of Vibrio parahaemolyticus which phenomenon was also found by the cells of strain 00-Bat-05.
内容記述言語 en
要約
内容記述タイプ Other
内容記述 連合農学研究科博士論文(水産学) ; 学位取得日: 平成20年3月14日
内容記述言語 ja
作成日
日付 2008-03-14
日付タイプ Issued
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
NDC
主題Scheme NDC
主題 660
ファイル(説明)
内容記述 学位論文の要旨, 学位論文本文
公開者・出版者
出版者 鹿児島大学
出版者言語 ja
公開者・出版者
出版者 Kagoshima University
出版者言語 en
学位記番号
学位記番号 連研第611号
学位名
学位名の言語 ja
学位名 博士(水産学)
学位名
学位名の言語 en
学位名 Doctor of Philosophy in Fisheries Science
学位授与機関名
学位授与機関識別子Scheme kakenhi
学位授与機関識別子 17701
学位授与機関名の言語 ja
学位授与機関名 鹿児島大学
学位授与年月日
学位授与年月日 2008-03-14
学位授与番号
学位授与番号 甲連研第611号
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