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  1. 掲載誌一覧
  2. 鹿児島大学水産学部紀要
  3. vol.23
  1. 水産学部
  2. 水産学部・紀要論文

海底泥より分離したテンペレート・ファージ系の性状

http://hdl.handle.net/10232/13728
http://hdl.handle.net/10232/13728
ddc0e843-ecfa-429f-9189-3740261218ef
名前 / ファイル ライセンス アクション
AN00040498_v23_p137-148.pdf AN00040498_v23_p137-148.pdf (6.4 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2015-06-17
タイトル
タイトル 海底泥より分離したテンペレート・ファージ系の性状
タイトルよみ
タイトルよみ カイテイデイ ヨリ ブンリ シタ テンペレート・ファージケイ ノ セイジョウ
別言語のタイトル
その他のタイトル Preliminary Characteristics of a Temperate Phage System Isolated from Marine Mud
著者 日高, 富男

× 日高, 富男

WEKO 102421

日高, 富男

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白浜, 豊宏

× 白浜, 豊宏

WEKO 102422

白浜, 豊宏

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著者よみ
姓名 ヒダカ, トミオ
著者よみ
姓名 シラハマ, トヨヒロ
別言語の著者
姓名 HIDAKA, Tomio
別言語の著者
姓名 SHIRAHAMA, Toyohiro
言語
言語 jpn
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ departmental bulletin paper
要約(Abstract)
内容記述タイプ Other
内容記述 A temperate phage designated 07T-12P was induced from a lysogenic strain (07T-12L) isolated from Kagoshima Bay mud. The lysogenic strain is a monotrichous polarly flagellated marine Pseudomonas sp. The phage 07T-12P give turbid plaque with diameter in 2-3mm on the sensitive strain (07T-12S) in duble agar layer plate. The electron micrograph of the phage particles shows that the structure of them has hexagonal and approximately 60 mμ in diameter head, and very small tail. The special character of the phage is to be very sensitive to heat and chloroform. When phage 07T-12P and strain 07T-12S were mixed at a MOI of 0.01 in sea water broth, there was 85-90 per-cent adsorption within 20 minutes at 25°C. The One-step growth curve of this phage shows that the latent period is 60 minutes, the rise period 35 minutes, and the average burst size is calculated to be 25 phage particles per infected cell. The phage yield was much lower. The cells of 07T-12L induced spontaneously the phage 07T-12P at a ratio of 0.8 per-cent of them. The phage induction rate of 07T-12L cells increased as high as 70-75 per-cent when they were irradiated ultraviolet light or treated with mitomycin C. On the other hand, when the phage 07T-12P was infected to 07T-12S cells at a MOI of 10, a 10-16 per-cent of the cells resulted in the lysogenic response and the others, a 84-90 per-cent of them resulted in the litic response. In this paper, the authors revealed the characteristics at the phases in the lysogenic cycle of the marine temperate phage system.
収録雑誌名 鹿児島大学水産学部紀要=Memoirs of Faculty of Fisheries Kagoshima University

巻 23, p. 137-148
作成日
日付 1974-12-25
ISSN
収録物識別子タイプ ISSN
ISSN 0453087X
NII書誌ID(雑誌)
収録物識別子タイプ NCID
NC ID AN00040498
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
NDC
主題Scheme NDC
主題 660
公開者・出版者
出版者 鹿児島大学
公開者よみ
公開者よみ カゴシマ ダイガク
公開者別名
公開者別名 Kagoshima University
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