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イヌZUBECA4プライマーによって増幅されるニホンタヌキマイクロサテライト座について
http://hdl.handle.net/10232/14443
http://hdl.handle.net/10232/14443f1250d8e-721a-4b77-a3d3-30d333b2bbdc
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00040104_v61n3_p41-46.pdf (246.5 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-07-03 | |||||
| タイトル | ||||||
| タイトル | Amplification of Microsatellites of Japanese Raccoon Dogs (Nyctereutes procyonoides viverrinus) with Primers for the Dog ZUBECA4 Locus | |||||
| タイトル言語 | en | |||||
| タイトル | ||||||
| タイトル | イヌZUBECA4プライマーによって増幅されるニホンタヌキマイクロサテライト座について | |||||
| タイトル言語 | ja | |||||
| 著者 |
林園, 典子
× 林園, 典子× 吾郷, 一利× 林, 敬人× 吾郷, 美保子× 小片, 守 |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | forensic genetics | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | DNA polymorphism | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | STR | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | microsatellite | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | dog ZUBECA4 primers | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Japanese raccoon dog | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The Japanese raccoon dog short tandem repeats (STRs, or microsatellites) were investigated using the primer set for the ZUBECA4 locus, one of the dog STR primers. The properties of Japanese raccoon dog PCR products were examined by electrophoresis and sequence analyses. Six alleles were detected by the electrophoresis analyses in nine Japanese raccoon dogs. The sequence analyses on the six alleles revealed that variants are based on the difference in the total number of repeats of GAAA and GCAA. The repeating units of Japanese raccoon dogs were simpler than those of dog alleles. The six alleles were designated as 12-16 and 18. Allele 15 and 18 were divided into 15^{12}, 15^{14}, 15^{15}, 18^{13} and 18^{14} sub-alleles by the times GAAA was repeated. Their frequencies were estimated to be: allele 12=0.10, allele 13=0.17, allele 14=0.17, allele 15^{12}=0.22, allele 15^{14}=0.06, allele 15^{15}=0.06, allele 16=0.06, allele 18^{13}=0.06 and allele 18^{14}=0.10. Heterozygosity was calculated to be 0.79 in STR alleles and 0.86 in sub-alleles. Further, new primers for Japanese raccoon dogs were developed based on the sequences of the Japanese raccoon dog alleles. This primer set was successfully amplified as a small-sized amplicon, which is more suitable for practical uses than dog primers. These findings indicated that the ZUBECA4 locus is a quite useful STR for species identification, individual identification, pedigree study and population study on Japanese raccoon dogs. |
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| 内容記述言語 | en | |||||
| 収録雑誌名 |
ja : 鹿児島大学医学雑誌 en : Medical journal of Kagoshima University 巻 61, 号 3, p. 41-46, 発行日 2010-01-01 |
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| 作成日 | ||||||
| 日付 | 2010-01-01 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| ISSN | 03685063 | |||||
| NII書誌ID（雑誌） | ||||||
| 収録物識別子タイプ | NCID | |||||
| NC ID | AN00040104 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 493 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 出版者言語 | en | |||||
