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The heart of bull frog perfused with the solution containing the extracts was arrested in systole. These seemed to be due to a saponin-like agent which was the common component of sea animals, sea stars and sea cucumbers. In the isolated bull frog nerve-muscle preparation, the extracts abolished nerve stimulated contractions but did not affect the contractions produced by\ndirect stimulations of the muscle. In addition, the extracts caused a significant increase in miniature endplate potentials (MEPPs) frequency at a neuromuscular junction and this effect was reversible. Both activities on the muscle contraction and on the MEPPs frequency were separated from the saponin-like agent by a dialysis or gelfiltrations on Sephadex G-10, G-25 and Bio Gel P-4. 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