{"_buckets": {"deposit": "4ede972e-11e8-43ed-ae17-6a06a060c3cc"}, "_deposit": {"created_by": 3, "id": "8995", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "8995"}, "status": "published"}, "_oai": {"id": "oai:ir.kagoshima-u.ac.jp:00008995", "sets": ["80"]}, "author_link": ["28013", "118024", "118023"], "item_8_alternative_title_18": {"attribute_name": "別言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Analysis of Cell response by low dose irradiation"}]}, "item_8_alternative_title_19": {"attribute_name": "タイトルよみ", "attribute_value_mlt": [{"subitem_alternative_title": "テイセンリョウ ホウシャセン ニヨル サイボウ ノ ホウシャセン テキオウ オウトウ メカニズム ノ カイメイ"}]}, "item_8_date_6": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2014-06-17"}]}, "item_8_description_4": {"attribute_name": "要約(Abstract)", "attribute_value_mlt": [{"subitem_description": "2012-2013年度科学研究費助成事業（若手研究（B））研究成果報告書　課題番号：24792003　研究代表者：富田和男（鹿児島大学・医歯（薬）学総合研究科・助教）", "subitem_description_type": "Other"}, {"subitem_description": "細胞死などに有意な変化を検出できない0.1mGy-100mGyといったごく低線量の放射線照射による細胞応答について、定量PCR法を用いて遺伝子発現変化を調べた。その結果、神経細胞芽腫NB-1細胞において、p53やMnSODなどの遺伝子発現が有意に変化することが明らかとなった。また、ミトコンドリアDNA突然変異を従来のRFLP法とSSCP法、および新しい方法であるTaqMan probeを用いた方法で比較検討した。その結果、TaqMan probeを用いた方法が、最も簡便に、かつ従来法より再現性良く突然変異を検出できることがわかった。", "subitem_description_type": "Other"}, {"subitem_description": "The cell response of low dose (0.1-100mGy) irradiation was investigated. Quantitative PCR was carried out whether specific gene expression change or not. As a result, 0.1 to 100mGy irradiation, that dose do not affect apoptosis ratio significantly, cause specific gene expression change such as p53, MnSOD in neuroblastoma cell line NB-1. Furthermore, to detect mitochondria DNA mutation, RELP, SSCP and TaqMan based QPCR was performed. As a result, The TaqMan probe method was the easiest and most reproductive among them.", "subitem_description_type": "Other"}]}, "item_8_full_name_2": {"attribute_name": "著者よみ", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "118023", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "トミタ, カズオ"}]}]}, "item_8_full_name_3": {"attribute_name": "別言語の著者", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "118024", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "TOMITA, Kazuo"}]}]}, "item_8_publisher_23": {"attribute_name": "公開者・出版者", "attribute_value_mlt": [{"subitem_publisher": "鹿児島大学"}]}, "item_8_subject_15": {"attribute_name": "NDC", "attribute_value_mlt": [{"subitem_subject": "492", "subitem_subject_scheme": "NDC"}]}, "item_8_text_24": {"attribute_name": "公開者よみ", "attribute_value_mlt": [{"subitem_text_value": "カゴシマ ダイガク"}]}, "item_8_text_25": {"attribute_name": "公開者別名", "attribute_value_mlt": [{"subitem_text_value": "Kagoshima University"}]}, "item_8_text_30": {"attribute_name": "NIIタイプ", "attribute_value_mlt": [{"subitem_text_value": "Research Paper"}]}, "item_8_text_41": {"attribute_name": "科研費番号 ", "attribute_value_mlt": [{"subitem_text_value": "24792003"}]}, "item_8_version_type_14": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "富田, 和男"}], "nameIdentifiers": [{"nameIdentifier": "28013", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000060347094", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-10-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "24792003_富田和男.pdf", "filesize": [{"value": "550.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 550000.0, "url": {"label": "24792003_富田和男.pdf", "url": "https://ir.kagoshima-u.ac.jp/record/8995/files/24792003_富田和男.pdf"}, "version_id": "cc7ceb5a-6586-4396-92e2-d8af86c8ce87"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "病態科学系歯学", "subitem_subject_scheme": "Other"}, {"subitem_subject": "歯科放射線学", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "低線量放射線による細胞の放射線適応応答メカニズムの解明", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "低線量放射線による細胞の放射線適応応答メカニズムの解明"}]}, "item_type_id": "8", "owner": "3", "path": ["80"], "permalink_uri": "http://hdl.handle.net/10232/23860", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-05-19"}, "publish_date": "2015-05-19", "publish_status": "0", "recid": "8995", "relation": {}, "relation_version_is_last": true, "title": ["低線量放射線による細胞の放射線適応応答メカニズムの解明"], "weko_shared_id": 3}