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Characterization of cytoplasmic fibril structures found in gliding cells of Saprospira sp.
http://hdl.handle.net/10232/297
http://hdl.handle.net/10232/2979fe6f04d-956b-489d-837f-8aa6f39a3592
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
reposit0002.pdf (2.7 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-07-18 | |||||
| タイトル | ||||||
| タイトル | Characterization of cytoplasmic fibril structures found in gliding cells of Saprospira sp. | |||||
| タイトル言語 | en | |||||
| 著者 |
Furusawa, Gou
× Furusawa, Gou× Yoshikawa, Takeshi× Takano, Yoshitaka× Mise, Kazuyuki× Furusawa, Iwao× Okuno, Tetsuro× Sakata, Taizo |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Saprospira | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | gliding motility | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Saprospira cytoplasmic fibril protein | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The cytoplasmic fibril structures of Saprospira sp. strain SS98-5 grown on a low-nutrient agar medium were purified from cell lysates treated with Triton X-100 and were observed by electron microscopy to be about 7 nm in width and 200-300 nm in length. SDS-PAGE of the fibril structures exhibited a single protein band with a molecular mass of 61 kDa. A Saprospira cytoplasmic fibril protein (SCFP), which is a subunit of the fibril structures, was di gested with trypsin to oligopeptides and analyzed for amino acid sequences. A partial nucleotide sequence of the SCFP gene was determined after PCR using primers designated from the amino acid sequences of the oligopeptides. SCFP gene including DNA fragments were detected by Southern hybridization using the PCR product for an SCFP gene as a probe and were cloned to determine whole nucleotide sequences. The SCFP gene indicated relatively higher similarity to conserved hypothetical phage tail sheath proteins. A Western immunoblotting analysis showed that SCFP was signif icantly expressed in gliding cells as compared with nongliding cells. The above findings with the previously reported results suggest that the cytoplasmic fibril structures are possibly related to the gliding motility of Saprospira sp. strain SS98-5. |
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| 内容記述言語 | en | |||||
| 収録雑誌名 |
en : Canadian journal of microbiology 巻 51, 号 10, p. 875-880, 発行日 2005 |
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| 作成日 | ||||||
| 日付 | 2005-01-01 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| ISSN | 00084166 | |||||
| NII書誌ID（雑誌） | ||||||
| 収録物識別子タイプ | NCID | |||||
| NC ID | AA00597703 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| DOI | https://doi.org/10.1139/W05-081 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 663 | |||||
| NII subject | ||||||
| 主題言語 | ja | |||||
| 主題Scheme | Other | |||||
| 主題 | 自然科学 | |||||
| 公開者・出版者 | ||||||
| 出版者 | National Research Council Canada | |||||
| 出版者言語 | en | |||||
