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Effect of Simulated Microgravity on Murine Melanoma Cells : Altered Tumor Cell and Tumorigenicity
http://hdl.handle.net/10232/2836
http://hdl.handle.net/10232/283646f69cd4-6e46-47db-b6fa-c2017d3a8ff6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004161865.pdf (675.9 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-04-24 | |||||
| タイトル | ||||||
| タイトル | Effect of Simulated Microgravity on Murine Melanoma Cells : Altered Tumor Cell and Tumorigenicity | |||||
| タイトル言語 | en | |||||
| 著者 |
TAGA, Masaki
× TAGA, Masaki× KANOUCHI, Hiroaki× OKA, Tatsuzo |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Tumor growth | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | tumorigenicity | |||||
| キーワード | ||||||
| 主題言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | microgravity | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Short-term or long-term space flight induces numerous biological stressors that affect several human physiological systems. The physical factors and their physiologic stressors cause several system dysfunctions. The incidence of tumor growth and carcinogenesis in microgravity is yet unknown. Hence, we investigated the effects of simulated microgravity on tumor growth and tumorigenicity using ground-based in vitro and in vivo models. Murine B16-F10 melanoma cells were cultured in a rotating wall vessel bioreactor (BIO), designed by NASA to simulate some aspects of microgravity, and a tissue culture flask (FL). We then measured cell growth, melanin production and apoptosis. After 48 hours of culture in FL and BIO, cells were inoculated subcutaneously in C57BL/6 mice syngeneic hosts for B16-F10 tumor cells. The growth of BIO cells decreased to 50% (p<0.01), and doubling time increased. Melanin production, a marker of differentiation, increased to 150% (p<0.05) in BIO. Flow cytometry analysis showed that there was increase in the percentage of apoptotic cells in the BIO when compared with that in the FL. When BIO cultured melanoma cells were inoculated subcutaneously in mice there was significant increase in tumorigenicity (p<.001) as compared to FL cells. Our results indicate that simulated microgravity may have altered the tumor cell characteristics and enhanced the invasive property. It is possible that the microgravity analog culture environment may have selected highly tumorigenic cells for survival despite the decreased overall growth in the microgravity analog. | |||||
| 内容記述言語 | en | |||||
| 収録雑誌名 |
en : Memoirs of the Faculty of Agriculture, Kagoshima University 巻 40, p. 25-33, 発行日 2005-02-20 |
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| 作成日 | ||||||
| 日付 | 2005-02-20 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| ISSN | 04530853 | |||||
| NII書誌ID（雑誌） | ||||||
| 収録物識別子タイプ | NCID | |||||
| NC ID | AA00732376 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 610 | |||||
| NIIsubject | ||||||
| 主題言語 | ja | |||||
| 主題Scheme | Other | |||||
| 主題 | 農学 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 出版者言語 | en | |||||
