WEKO3
アイテム
ビューレット試薬による魚肉蛋白質の迅速定量I : 呈色反応の促進と発色液の濁りの除去
http://hdl.handle.net/10232/13907
http://hdl.handle.net/10232/139077a3c0249-8098-4f03-9798-941ed649a6cb
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN00040498_v6_p119-124.pdf (3.2 MB)
|
|
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-05-15 | |||||
| タイトル | ||||||
| タイトル | ビューレット試薬による魚肉蛋白質の迅速定量I : 呈色反応の促進と発色液の濁りの除去 | |||||
| タイトル言語 | ja | |||||
| タイトル | ||||||
| タイトル | Rapid Determination of Protein in Fish Muscle through Biuret Reagent I : Acceleration of Color Reaction and Removal of Turbid Substances Yielded in the Color Solution | |||||
| タイトル言語 | en | |||||
| 著者 |
大城, 善太郎
× 大城, 善太郎 |
|||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 要約 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | A rapid method of the colorimetric estimation of the modicum of protein in fish muscle under the use of biuret reagent (modified Sol's reagent) was put under examination, with the following procedure ascertained. After taking 5ml. of sample into a colorimeter tube in which 0.5 to 4.5mg. protein-nitogen was contained, 5ml. of biuret reagent was added. Then, after a well mixing, it was heated for 1 minute at 100°C on a water bath, to accelerate the color development. Although, the maximum color development required 1 minute, the development itself occurred almost immediately. Then, the colorimeter tube was cooled in a running water for about 1 minute. And with the purpose of removing the turbid substances yielded, 1 ml of reagent mixture (20% MgSO_4・7H_2O - 35% ZnSO_4・7H_2O) was added to the above mentioned colored solution. Then, those substances were adsorbed by the colloidal magnesium hydroxide prepared with magnesium sulfate and sodium hydroxide in biuret colored solution, in this case the former was added after being well mixed by the vigorous shaking for a spell of several seconds. The mixed solution was filtered after 2 minutes standing. The optical density of the filtrate was read at the wave length of 530mμ. By the proposed method, the determination of protein was to be done with comparatively correct accuracy, the error, compared with that of Kjeldahl value, being to be kept within the range of ±2%. | |||||
| 内容記述言語 | en | |||||
| 収録雑誌名 |
ja : 鹿児島大学水産学部紀要 en : Memoirs of Faculty of Fisheries Kagoshima University 巻 6, p. 119-124, 発行日 1958-03-15 |
|||||
| 作成日 | ||||||
| 日付 | 1958-03-15 | |||||
| 日付タイプ | Issued | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| ISSN | 0453087X | |||||
| NII書誌ID（雑誌） | ||||||
| 収録物識別子タイプ | NCID | |||||
| NC ID | AN00040498 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 660 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 鹿児島大学 | |||||
| 出版者言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Kagoshima University | |||||
| 出版者言語 | en | |||||
