{"_buckets": {"deposit": "f97498d6-606c-44cf-a4f3-f5ee4967be5f"}, "_deposit": {"created_by": 3, "id": "3796", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "3796"}, "status": "published"}, "_oai": {"id": "oai:ir.kagoshima-u.ac.jp:00003796", "sets": ["78"]}, "author_link": ["116881", "116882", "116880", "116884", "116885", "116883", "116879"], "item_6_biblio_info_5": {"attribute_name": "収録雑誌名", "attribute_value_mlt": [{"bibliographicPageEnd": "35", "bibliographicPageStart": "27", "bibliographicVolumeNumber": "5", "bibliographic_titles": [{"bibliographic_title": "Dendritic cells"}]}]}, "item_6_date_6": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "1995-01-01"}]}, "item_6_description_4": {"attribute_name": "要約(Abstract)", "attribute_value_mlt": [{"subitem_description": "In order to detect histochemically signals of human T-cell leukemia virus type 1 (HTLV-1) proviral DNA and its proviral DNA in MT-2 cells, we made highly biotinylated  probes corresponding to parts of HTLV-1 proviral DNA by means of 2 times polymerase chain reaction (PCR) and improved the procedures of DNA-RNA and DNA DNA cold in-situ-hybridization (cISH) for HTLV-1. We produced 7 highly biotinylated double-stranded DNA probes for the long terminal repeat, gag-pol region, env region  and pX tax and rex regions of HTLV-1. DNA-RNA cISH of these probes was established, evaluating the effect of 17% polyvinyl alcohol solvent for reducing non-specific alkaline phosphatase reaction in the process of visualizing hybridized probe and suggesting the necessary prehybridization incubation with hybridization buffer containing salmon DNA for diminishing non-specific binding of probes with tissue. On the other hand, DNA-DNA cISH of a cocktail of these 7 probes could show only too faint dot-like stain in nuclei to be applied to a study of HTLV-l-related lesions, denaturing DNA in sections and probes in aluminum box for 10 min at 200℃, incubating the sections with probes for hybridization for 3 days at 45℃ and performing overnight alkaline phosphatase reaction by using 17 % polyvinyl alcohol solvent after streptavidine-biotinylated alkaline phosphatase system to detect the hybridized probes.", "subitem_description_type": "Other"}]}, "item_6_publisher_23": {"attribute_name": "公開者・出版者", "attribute_value_mlt": [{"subitem_publisher": "日本樹状細胞研究会"}]}, "item_6_source_id_9": {"attribute_name": "NII書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11039557", "subitem_source_identifier_type": "NCID"}]}, "item_6_subject_15": {"attribute_name": "NDC", "attribute_value_mlt": [{"subitem_subject": "490", "subitem_subject_scheme": "NDC"}]}, "item_6_subject_20": {"attribute_name": "NIIsubject", "attribute_value_mlt": [{"subitem_subject": "医学", "subitem_subject_scheme": "Other"}]}, "item_6_text_25": {"attribute_name": "公開者別名", "attribute_value_mlt": [{"subitem_text_value": "Japanese Dendritic Cells Society"}]}, "item_6_text_30": {"attribute_name": "NIIタイプ", "attribute_value_mlt": [{"subitem_text_value": "Journal Article"}]}, "item_6_version_type_14": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hasui, Kazuhisa"}], "nameIdentifiers": [{"nameIdentifier": "116879", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato, Eiichi"}], "nameIdentifiers": [{"nameIdentifier": "116880", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sueyoshi, Kazunobu"}], "nameIdentifiers": [{"nameIdentifier": "116881", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitajima, Shinichi"}], "nameIdentifiers": [{"nameIdentifier": "116882", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Goto, Masamitsu"}], "nameIdentifiers": [{"nameIdentifier": "116883", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakamura, Takao"}], "nameIdentifiers": [{"nameIdentifier": "116884", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsumoto, Daiten"}], "nameIdentifiers": [{"nameIdentifier": "116885", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-10-27"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "DC(Hasui)1995.pdf", "filesize": [{"value": "3.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 3400000.0, "url": {"label": "DC(Hasui)1995.pdf", "url": "https://ir.kagoshima-u.ac.jp/record/3796/files/DC(Hasui)1995.pdf"}, "version_id": "621619bf-1001-4bc0-85be-80b36f55cde2"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "HTLV-1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DNA-DNA cold in-situ-hybridization", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DNA-RNA cold-in-situ-hybridization", "subitem_subject_scheme": "Other"}, {"subitem_subject": "MT-2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "biotinylated double-stranded DNA probes", "subitem_subject_scheme": "Other"}, {"subitem_subject": "polymerase chain reaction", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Cold in-situ-hybridization of human T-cell leukemia virus type 1: Production of biotinylated probes by polymerase chain reaction and improvement of procedures", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Cold in-situ-hybridization of human T-cell leukemia virus type 1: Production of biotinylated probes by polymerase chain reaction and improvement of procedures"}]}, "item_type_id": "6", "owner": "3", "path": ["78"], "permalink_uri": "http://hdl.handle.net/10232/3739", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-04-13"}, "publish_date": "2010-04-13", "publish_status": "0", "recid": "3796", "relation": {}, "relation_version_is_last": true, "title": ["Cold in-situ-hybridization of human T-cell leukemia virus type 1: Production of biotinylated probes by polymerase chain reaction and improvement of procedures"], "weko_shared_id": 3}