Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2010-04-13 |
タイトル |
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タイトル言語 |
en |
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タイトル |
Cold in-situ-hybridization of human T-cell leukemia virus type 1: Production of biotinylated probes by polymerase chain reaction and improvement of procedures |
著者 |
Hasui, Kazuhisa
Sato, Eiichi
Sueyoshi, Kazunobu
Kitajima, Shinichi
Goto, Masamitsu
Nakamura, Takao
Matsumoto, Daiten
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言語 |
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言語 |
eng |
キーワード |
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主題言語 |
en |
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主題Scheme |
Other |
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主題 |
HTLV-1 |
キーワード |
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主題言語 |
en |
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主題Scheme |
Other |
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主題 |
DNA-DNA cold in-situ-hybridization |
キーワード |
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主題言語 |
en |
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主題Scheme |
Other |
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主題 |
DNA-RNA cold-in-situ-hybridization |
キーワード |
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主題言語 |
en |
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主題Scheme |
Other |
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主題 |
MT-2 |
キーワード |
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主題言語 |
en |
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主題Scheme |
Other |
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主題 |
biotinylated double-stranded DNA probes |
キーワード |
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主題言語 |
en |
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主題Scheme |
Other |
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主題 |
polymerase chain reaction |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
要約(Abstract) |
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内容記述タイプ |
Other |
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内容記述 |
In order to detect histochemically signals of human T-cell leukemia virus type 1 (HTLV-1) proviral DNA and its proviral DNA in MT-2 cells, we made highly biotinylated probes corresponding to parts of HTLV-1 proviral DNA by means of 2 times polymerase chain reaction (PCR) and improved the procedures of DNA-RNA and DNA DNA cold in-situ-hybridization (cISH) for HTLV-1. We produced 7 highly biotinylated double-stranded DNA probes for the long terminal repeat, gag-pol region, env region and pX tax and rex regions of HTLV-1. DNA-RNA cISH of these probes was established, evaluating the effect of 17% polyvinyl alcohol solvent for reducing non-specific alkaline phosphatase reaction in the process of visualizing hybridized probe and suggesting the necessary prehybridization incubation with hybridization buffer containing salmon DNA for diminishing non-specific binding of probes with tissue. On the other hand, DNA-DNA cISH of a cocktail of these 7 probes could show only too faint dot-like stain in nuclei to be applied to a study of HTLV-l-related lesions, denaturing DNA in sections and probes in aluminum box for 10 min at 200℃, incubating the sections with probes for hybridization for 3 days at 45℃ and performing overnight alkaline phosphatase reaction by using 17 % polyvinyl alcohol solvent after streptavidine-biotinylated alkaline phosphatase system to detect the hybridized probes. |
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内容記述言語 |
en |
収録雑誌名 |
en : Dendritic cells
巻 5,
p. 27-35,
発行日 1995-01-01
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作成日 |
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日付 |
1995-01-01 |
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日付タイプ |
Issued |
NII書誌ID(雑誌) |
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収録物識別子タイプ |
NCID |
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NC ID |
AA11039557 |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
NDC |
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主題Scheme |
NDC |
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主題 |
490 |
NIIsubject |
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主題言語 |
ja |
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主題Scheme |
Other |
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主題 |
医学 |
公開者・出版者 |
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出版者言語 |
ja |
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出版者 |
日本樹状細胞研究会 |
公開者・出版者 |
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出版者言語 |
en |
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出版者 |
Japanese Dendritic Cells Society |