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Cold in-situ-hybridization of human T-cell leukemia virus type 1: Production of biotinylated probes by polymerase chain reaction and improvement of procedures
http://hdl.handle.net/10232/3739
http://hdl.handle.net/10232/37398ad04069-7623-4186-a679-313b1cf60c96
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
DC(Hasui)1995.pdf (3.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2010-04-13 | |||||
| タイトル | ||||||
| タイトル | Cold in-situ-hybridization of human T-cell leukemia virus type 1: Production of biotinylated probes by polymerase chain reaction and improvement of procedures | |||||
| 言語 | en | |||||
| 著者 |
Hasui, Kazuhisa
× Hasui, Kazuhisa× Sato, Eiichi× Sueyoshi, Kazunobu× Kitajima, Shinichi× Goto, Masamitsu× Nakamura, Takao× Matsumoto, Daiten |
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| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | HTLV-1 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | DNA-DNA cold in-situ-hybridization | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | DNA-RNA cold-in-situ-hybridization | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | MT-2 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | biotinylated double-stranded DNA probes | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | polymerase chain reaction | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 要約(Abstract) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | In order to detect histochemically signals of human T-cell leukemia virus type 1 (HTLV-1) proviral DNA and its proviral DNA in MT-2 cells, we made highly biotinylated probes corresponding to parts of HTLV-1 proviral DNA by means of 2 times polymerase chain reaction (PCR) and improved the procedures of DNA-RNA and DNA DNA cold in-situ-hybridization (cISH) for HTLV-1. We produced 7 highly biotinylated double-stranded DNA probes for the long terminal repeat, gag-pol region, env region and pX tax and rex regions of HTLV-1. DNA-RNA cISH of these probes was established, evaluating the effect of 17% polyvinyl alcohol solvent for reducing non-specific alkaline phosphatase reaction in the process of visualizing hybridized probe and suggesting the necessary prehybridization incubation with hybridization buffer containing salmon DNA for diminishing non-specific binding of probes with tissue. On the other hand, DNA-DNA cISH of a cocktail of these 7 probes could show only too faint dot-like stain in nuclei to be applied to a study of HTLV-l-related lesions, denaturing DNA in sections and probes in aluminum box for 10 min at 200℃, incubating the sections with probes for hybridization for 3 days at 45℃ and performing overnight alkaline phosphatase reaction by using 17 % polyvinyl alcohol solvent after streptavidine-biotinylated alkaline phosphatase system to detect the hybridized probes. | |||||
| 言語 | en | |||||
| 収録雑誌名 |
en : Dendritic cells 巻 5, p. 27-35, 発行日 1995-01-01 |
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| 作成日 | ||||||
| 日付 | 1995-01-01 | |||||
| 日付タイプ | Issued | |||||
| NII書誌ID（雑誌） | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11039557 | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| NDC | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 490 | |||||
| NIIsubject | ||||||
| 言語 | ja | |||||
| 主題Scheme | Other | |||||
| 主題 | 医学 | |||||
| 公開者・出版者 | ||||||
| 出版者 | 日本樹状細胞研究会 | |||||
| 言語 | ja | |||||
| 公開者・出版者 | ||||||
| 出版者 | Japanese Dendritic Cells Society | |||||
| 言語 | en | |||||
