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  1. 理工学研究科
  2. 理工学研究科・博士論文

AgrobacteriumとRhizobium間を移動する巨大プラスミドと共生能発現

http://hdl.handle.net/10232/4957
http://hdl.handle.net/10232/4957
1ade67f2-b503-4ca9-bc43-772bc3649e24
名前 / ファイル ライセンス アクション
rikouken285.pdf rikouken285.pdf (142.9 kB)
thesis.pdf thesis.pdf (575.2 kB)
Figures.pdf Figures.pdf (8.4 MB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2015-02-18
タイトル
タイトル AgrobacteriumとRhizobium間を移動する巨大プラスミドと共生能発現
タイトル
タイトル言語 en
タイトル Intergeneric transfer of a symbiotic plasmid between Agrobacterium and Rhizobium and expression of its symbiotic ability with leguminous plants
タイトルよみ
タイトルよみ Agrobacterium ト Rhizobium カン オ イドウ スル キョダイ プラスミド ト キョウセイノウ ハツゲン
著者 中務, 弘基

× 中務, 弘基

WEKO 125838

中務, 弘基

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著者よみ
姓名 ナカツカサ, ヒロキ
別言語の著者
姓名 NAKATSUKASA, Hiroki
言語
言語 jpn
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_db06
資源タイプ doctoral thesis
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
要約(Abstract)
内容記述タイプ Other
内容記述 The symbiotic plasmid (pSym) of Rhizobium leguminosarum bv. trifolii 4S5, which carries Tn5-mob, was successfully transferred into Agrobacterium tumefaciens A136 by using a conjugation method. The resulting transconjugants induced the development of ineffective nitrogen-fixing nodules on the roots of white clover seedlings. Depending on the manner in which the pSym was retained, the transconjugants were divided into two groups of strains, Afp and Afcs. pSym was retained as a plasmid in the Afp strains but was integrated into int gene encoding phage-related integrase on the linear chromosome of A. tumefaciens A136 in strain Afcs1 (one of the Afcs strains) to form a symbiosis island. Conjugation was performed between strain Afcs1 and R. leguminosarum bv. trifolii H1 (a pSym-cured derivative of wild-type strain 4S), and the Rhizobium H1tr strains were screened as transconjugants. Eighteen of the H1tr strains induced effective nitrogen-fixing nodules on the roots of the host plants. pSym was transferred into all of the transconjugants, except for strain H1tr1, at the same size as pSym of strain 4S5. In strain H1tr1, pSym was integrated into the chromosome as a symbiosis island. These data suggest that pSym can exist among Rhizobium and Agrobacterium strains both as a plasmid and as a symbiosis island with transposon mediation.
要約(Abstract)
内容記述タイプ Other
内容記述 理工学研究科博士論文(理学) ; 学位取得日: 平成20年3月25日
作成日
日付 2008-03-25
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
NDC
主題Scheme NDC
主題 460
ファイル(説明)
内容記述タイプ Other
内容記述 学位論文の要旨
ファイル(説明)
内容記述タイプ Other
内容記述 博士論文全文
公開者・出版者
出版者 鹿児島大学
公開者よみ
公開者よみ カゴシマ ダイガク
公開者別名
公開者別名 Kagoshima University
学位記番号
理工研第285号
学位名
学位名 博士(理学)Doctor of Philosophy in Science
学位授与機関
学位授与機関識別子Scheme kakenhi
学位授与機関識別子 17701
学位授与機関名 鹿児島大学
学位授与年月日
学位授与年月日 2008-03-25
学位授与番号
学位授与番号 甲理工研第285号
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