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AgrobacteriumとRhizobium間を移動する巨大プラスミドと共生能発現
http://hdl.handle.net/10232/4957
http://hdl.handle.net/10232/49571ade67f2-b503-4ca9-bc43-772bc3649e24
名前 / ファイル | ライセンス | アクション |
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2015-02-18 | |||||
タイトル | ||||||
タイトル | AgrobacteriumとRhizobium間を移動する巨大プラスミドと共生能発現 | |||||
タイトル | ||||||
タイトル言語 | en | |||||
タイトル | Intergeneric transfer of a symbiotic plasmid between Agrobacterium and Rhizobium and expression of its symbiotic ability with leguminous plants | |||||
タイトルよみ | ||||||
タイトルよみ | Agrobacterium ト Rhizobium カン オ イドウ スル キョダイ プラスミド ト キョウセイノウ ハツゲン | |||||
著者 |
中務, 弘基
× 中務, 弘基 |
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著者よみ | ||||||
姓名 | ナカツカサ, ヒロキ | |||||
別言語の著者 | ||||||
姓名 | NAKATSUKASA, Hiroki | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
要約(Abstract) | ||||||
内容記述タイプ | Other | |||||
内容記述 | The symbiotic plasmid (pSym) of Rhizobium leguminosarum bv. trifolii 4S5, which carries Tn5-mob, was successfully transferred into Agrobacterium tumefaciens A136 by using a conjugation method. The resulting transconjugants induced the development of ineffective nitrogen-fixing nodules on the roots of white clover seedlings. Depending on the manner in which the pSym was retained, the transconjugants were divided into two groups of strains, Afp and Afcs. pSym was retained as a plasmid in the Afp strains but was integrated into int gene encoding phage-related integrase on the linear chromosome of A. tumefaciens A136 in strain Afcs1 (one of the Afcs strains) to form a symbiosis island. Conjugation was performed between strain Afcs1 and R. leguminosarum bv. trifolii H1 (a pSym-cured derivative of wild-type strain 4S), and the Rhizobium H1tr strains were screened as transconjugants. Eighteen of the H1tr strains induced effective nitrogen-fixing nodules on the roots of the host plants. pSym was transferred into all of the transconjugants, except for strain H1tr1, at the same size as pSym of strain 4S5. In strain H1tr1, pSym was integrated into the chromosome as a symbiosis island. These data suggest that pSym can exist among Rhizobium and Agrobacterium strains both as a plasmid and as a symbiosis island with transposon mediation. | |||||
要約(Abstract) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 理工学研究科博士論文(理学) ; 学位取得日: 平成20年3月25日 | |||||
作成日 | ||||||
日付 | 2008-03-25 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
NDC | ||||||
主題Scheme | NDC | |||||
主題 | 460 | |||||
ファイル(説明) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 学位論文の要旨 | |||||
ファイル(説明) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 博士論文全文 | |||||
公開者・出版者 | ||||||
出版者 | 鹿児島大学 | |||||
公開者よみ | ||||||
公開者よみ | カゴシマ ダイガク | |||||
公開者別名 | ||||||
公開者別名 | Kagoshima University | |||||
学位記番号 | ||||||
理工研第285号 | ||||||
学位名 | ||||||
学位名 | 博士(理学)Doctor of Philosophy in Science | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17701 | |||||
学位授与機関名 | 鹿児島大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2008-03-25 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲理工研第285号 |