{"_buckets": {"deposit": "41c79ff1-9363-4d0c-acfd-21c910040f6f"}, "_deposit": {"created_by": 3, "id": "9008", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "9008"}, "status": "published"}, "_oai": {"id": "oai:ir.kagoshima-u.ac.jp:00009008", "sets": ["80"]}, "author_link": ["118033", "118034", "14913"], "item_8_alternative_title_18": {"attribute_name": "別言語のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Molecular analysis on the intercellular signaling relating to the bone destruction of ameloblastoma"}]}, "item_8_alternative_title_19": {"attribute_name": "タイトルよみ", "attribute_value_mlt": [{"subitem_alternative_title": "エナメル ジョウヒシュ ニヨル コツ ハカイ・ シンジュン ニ カカワル サイボウカン シグナル ノ カイメイ ト チリョウ センリャク エノ テンカイ"}]}, "item_8_date_6": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2014-06-06"}]}, "item_8_description_4": {"attribute_name": "要約(Abstract)", "attribute_value_mlt": [{"subitem_description": "2011-2013年度科学研究費助成事業（基盤研究（C））研究成果報告書　課題番号：23592929　研究代表者：中村典史（鹿児島大学・医歯（薬）学総合研究科・教授）", "subitem_description_type": "Other"}, {"subitem_description": "口腔粘膜上皮細胞由来の不死化細胞株（MOE-1）と濾胞型エナメル上皮腫細胞由来の不死化細胞株（AM-3）を作成し、既存の網状型エナメル上皮腫由来不死化細胞株AM-1と生物学的特性を比較した。AM-3はAm-1と類似の増殖態度、上皮系マーカーの発現を呈した。AM-1, AM-3はMOE-1よりMMP-2, -9の発現、Wnt関連遺伝子Wnt-5aやその受容体の発現が多かった。また、AM-3ではWnt-3a刺激によってMMP-9の発現が上昇することが観察された。これらのことから、エナメル上皮腫の局所浸潤性は、Wntシグナル経路を介したMMPの発現によって制御されていることが示唆された。", "subitem_description_type": "Other"}, {"subitem_description": "Immortalized epithelial cell line derived from oral mucosa  (MOE-1), as control, and immortalized ameloblastoma cell line derived from follicular-type ameloblastoma (AM-3) were established and their biological characteristics were compared to  AM-1, previously established immortalized ameloblastoma cells derived from prexiform-type ameloblastoma. AM-1 and AM-3 demonstrated positive reaction to the epithelial markers and negative to the mesenchymal one maintaining the morphological characteristics of the epithelial cell. Expressions of MMP-2 and MMP-9 were significantly higher in  AM-1 and AM-3 than  MOE-1. Wnt-5a and Wnt signaling receptors, LRP and Frizzled, of both  AM-1 and AM-3 were higher than  MOE-1 in the mRNA level. Furthermore, expression and activity of MMP-9 in AM-3 increased dose- and time-dependently when stimulated with Wnt-3a protein. These results suggested that local invasiveness of ameloblastoma may be regulated by MMPs expression through the Wnt signaling pathway.", "subitem_description_type": "Other"}]}, "item_8_full_name_2": {"attribute_name": "著者よみ", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "118033", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "ナカムラ, ノリフミ"}]}]}, "item_8_full_name_3": {"attribute_name": "別言語の著者", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "118034", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "NAKAMURA, Norifumi"}]}]}, "item_8_publisher_23": {"attribute_name": "公開者・出版者", "attribute_value_mlt": [{"subitem_publisher": "鹿児島大学"}]}, "item_8_subject_15": {"attribute_name": "NDC", "attribute_value_mlt": [{"subitem_subject": "493", "subitem_subject_scheme": "NDC"}]}, "item_8_text_24": {"attribute_name": "公開者よみ", "attribute_value_mlt": [{"subitem_text_value": "カゴシマ ダイガク"}]}, "item_8_text_25": {"attribute_name": "公開者別名", "attribute_value_mlt": [{"subitem_text_value": "Kagoshima University"}]}, "item_8_text_30": {"attribute_name": "NIIタイプ", "attribute_value_mlt": [{"subitem_text_value": "Research Paper"}]}, "item_8_text_41": {"attribute_name": "科研費番号 ", "attribute_value_mlt": [{"subitem_text_value": "23592929"}]}, "item_8_version_type_14": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "中村, 典史"}], "nameIdentifiers": [{"nameIdentifier": "14913", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000060217875", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-10-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "23592929_中村典史.pdf", "filesize": [{"value": "559.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 559900.0, "url": {"label": "23592929_中村典史.pdf", "url": "https://ir.kagoshima-u.ac.jp/record/9008/files/23592929_中村典史.pdf"}, "version_id": "65cfd509-969e-4231-a92f-e32704e6b533"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "口腔外科一般", "subitem_subject_scheme": "Other"}, {"subitem_subject": "エナメル上皮腫", "subitem_subject_scheme": "Other"}, {"subitem_subject": "歯原性腫瘍", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Wntシグナル", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "エナメル上皮腫による骨破壊・浸潤に関わる細胞間シグナルの解明と治療戦略への展開", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "エナメル上皮腫による骨破壊・浸潤に関わる細胞間シグナルの解明と治療戦略への展開"}]}, "item_type_id": "8", "owner": "3", "path": ["80"], "permalink_uri": "http://hdl.handle.net/10232/24106", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-05-22"}, "publish_date": "2015-05-22", "publish_status": "0", "recid": "9008", "relation": {}, "relation_version_is_last": true, "title": ["エナメル上皮腫による骨破壊・浸潤に関わる細胞間シグナルの解明と治療戦略への展開"], "weko_shared_id": 3}